TY - JOUR
T1 - Generation of pseudocontact shifts in protein NMR spectra with a genetically encoded cobalt(II)-binding amino acid
AU - Nguyen, Thi Hoang Duong
AU - Ozawa, Kiyoshi
AU - Stanton-Cook, Mitchell
AU - Barrow, Russell
AU - Huber, Thomas
AU - Otting, Gottfried
PY - 2011/1/17
Y1 - 2011/1/17
N2 - Playing tag: Incorporation of the genetically encoded amino acid bipyridylalanine into a protein creates a site-specific binding site for Co II. Pronounced pseudocontact shifts generated by the Co II ion in the NMR spectra delivers powerful long-range structure information for the facile analysis of proteins as well as protein-protein and protein-ligand complexes (see picture for a representation of the psuedocontact shift in the protein).
AB - Playing tag: Incorporation of the genetically encoded amino acid bipyridylalanine into a protein creates a site-specific binding site for Co II. Pronounced pseudocontact shifts generated by the Co II ion in the NMR spectra delivers powerful long-range structure information for the facile analysis of proteins as well as protein-protein and protein-ligand complexes (see picture for a representation of the psuedocontact shift in the protein).
KW - NMR spectroscopy
KW - cell-free protein synthesis
KW - protein modifications
KW - protein structures
KW - pseudocontact shifts
UR - http://www.scopus.com/inward/record.url?scp=78651389865&partnerID=8YFLogxK
U2 - 10.1002/anie.201005672
DO - 10.1002/anie.201005672
M3 - Article
SN - 1433-7851
VL - 50
SP - 692
EP - 694
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 3
ER -