Generation of Pseudocontact Shifts in Proteins with Lanthanides Using Small "Clickable" Nitrilotriacetic Acid and Iminodiacetic Acid Tags

Choy Loh, Bim Graham, Elwy Abdelkader Ali, Kellie L Tuck, Gottfried Otting

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Pseudocontact shifts (PCS) induced by paramagnetic lanthanide ions provide unique long-range structural information in nuclear magnetic resonance (NMR) spectra, but the site-specific attachment of lanthanide tags to proteins remains a challenge. Here we i
    Original languageEnglish
    Pages (from-to)5084-5092
    JournalChemistry, A European Journal
    Volume21
    Issue number13
    DOIs
    Publication statusPublished - 2015

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