TY - JOUR
T1 - Genome-wide characterization revealed role of NBS-LRR genes during powdery mildew infection in Vitis vinifera
AU - Goyal, Neetu
AU - Bhatia, Garima
AU - Sharma, Shailesh
AU - Garewal, Naina
AU - Upadhyay, Anuradha
AU - Upadhyay, Santosh Kumar
AU - Singh, Kashmir
N1 - Publisher Copyright:
© 2019 Elsevier Inc.
PY - 2020/1
Y1 - 2020/1
N2 - NBS-LRR comprises a large class of disease resistance (R) proteins that play a widespread role in plant protection against pathogens. In grapevine, powdery mildew cause significant losses in its productivity and efforts are being directed towards finding of resistance loci or genes imparting resistance/tolerance against such fungal diseases. In the present study, we performed genome-wide analysis of NBS-LRR genes during PM infection in grapevine. We identified 18, 23, 12, 16, 10, 10, 9, 20 and 14 differentially expressed NBS-LRR genes in response to PM infection in seven partially PM-resistant (DVIT3351.27, Husseine, Karadzhandal, Khalchili, Late vavilov, O34–16, Sochal) and 2 PM-susceptible (Carignan and Thompson seedless) V. vinifera accessions. Further, the identified sequences were characterized based on chromosomal locations, physicochemical properties, gene structure and motif analysis, and functional annotation by Gene Ontology (GO) mapping. The NBS-LRR genes responsive to powdery mildew could potentially be exploited to improve resistance in grapes.
AB - NBS-LRR comprises a large class of disease resistance (R) proteins that play a widespread role in plant protection against pathogens. In grapevine, powdery mildew cause significant losses in its productivity and efforts are being directed towards finding of resistance loci or genes imparting resistance/tolerance against such fungal diseases. In the present study, we performed genome-wide analysis of NBS-LRR genes during PM infection in grapevine. We identified 18, 23, 12, 16, 10, 10, 9, 20 and 14 differentially expressed NBS-LRR genes in response to PM infection in seven partially PM-resistant (DVIT3351.27, Husseine, Karadzhandal, Khalchili, Late vavilov, O34–16, Sochal) and 2 PM-susceptible (Carignan and Thompson seedless) V. vinifera accessions. Further, the identified sequences were characterized based on chromosomal locations, physicochemical properties, gene structure and motif analysis, and functional annotation by Gene Ontology (GO) mapping. The NBS-LRR genes responsive to powdery mildew could potentially be exploited to improve resistance in grapes.
KW - Expression
KW - Fungal infections
KW - Grapevine
KW - NBS-LRR genes
KW - Powdery mildew resistance
UR - http://www.scopus.com/inward/record.url?scp=85062101461&partnerID=8YFLogxK
U2 - 10.1016/j.ygeno.2019.02.011
DO - 10.1016/j.ygeno.2019.02.011
M3 - Article
SN - 0888-7543
VL - 112
SP - 312
EP - 322
JO - Genomics
JF - Genomics
IS - 1
ER -