Gradients of Photoinhibition in the interior of a leaf induced by Photoinhibition lights of different colours

Riichi Oguchi, Peter Douwstra, Fujita Takashi, Wah Chow, Ichiro Terashima

    Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

    Abstract

    Gradients of photoinhibition within a leaf caused by different color lights were studied to get more insight into the controversy whether photon absorption by chlorophyll or Mn is the primary cause of photoinhibition, suggested by the excess-energy hypothesis or the Mn hypothesis, respectively. We photoinhibited lincomycin-treated leaf-discs by white, blue, green or red light. A combination of a micro-fiber fluorometer, a fiber-thinning technique and a micro-manipulator enabled us to measure the chlorophyll fluorescence signals within a leaf. Gradients of photoinhibition were also compared with results from various conventional fluorometers to estimate their depth of signal detection. The photoinhibition was more severe in the descending order of blue, red and green light near the adaxial surface, and in the descending order of blue, green and red light in the deeper tissue, which is correlated with the absorption spectrum of chlorophyll and Mn, respe! ctively. These results cannot be explained by either hypothesis alone and strongly suggest that both mechanisms occur in photoinhibition. Fv/Fm values of photoinhibited leaves estimated with the conventional mechanisms were different from the whole tissue. This is because the depths, in which these systems detect fluorescence signals, differ depending on the wavelengths of measuring beam and detector.
    Original languageEnglish
    Title of host publicationAdvanced Topics in Science and Technology in China: Photosynthesis Research for Food, Fuel and Future: Proceedings of the 15th International Conference on Photosynthesis
    EditorsT Kuang, C Lu, L Zhang
    Place of PublicationGermany
    PublisherSpringer Science+Business Media
    Pages459-464
    Editionpeer reviewed
    ISBN (Print)9787308096942
    Publication statusPublished - 2012
    EventInternational Conference on Photosynthesis 2010 - Beijing China
    Duration: 1 Jan 2012 → …
    http://www.springer.com/life+sciences/plant+sciences/book/978-3-642-32033-0

    Conference

    ConferenceInternational Conference on Photosynthesis 2010
    Period1/01/12 → …
    OtherAugust 22-27 2010
    Internet address

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