Heteronuclear correlation experiments for the determination of one-bond coupling constants

Patrik Andersson; Kerstin Nordstrand; Maria Sunnerhagen; Edvards Liepinsh; Ivars Turovskis; Gottfried Otting

doi:10.1023/A:1008206212145

Heteronuclear correlation experiments for the determination of one-bond coupling constants

Patrik Andersson, Kerstin Nordstrand, Maria Sunnerhagen, Edvards Liepinsh, Ivars Turovskis, Gottfried Otting^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

Spin-state selective experiments, HSQC-α/β and CT-HMQC-α/β, are proposed for the simple and rapid measurement of scalar one-bond coupling constants in two-dimensional, ¹H-detected ¹⁵N-¹H or ¹³C-¹H correlation experiments based on HSQC and HMQC schemes. Pairs of subspectra are obtained, containing either the high-field or the low-field component of the doublet representing the one-bond coupling constant. The subspectral editing procedure retains the full sensitivity of HSQC and HMQC spectra recorded without heteronuclear decoupling during data acquisition, with a spectral resolution similar to that of decoupled spectra.

Original language	English
Pages (from-to)	445-450
Number of pages	6
Journal	Journal of Biomolecular NMR
Volume	11
Issue number	4
DOIs	https://doi.org/10.1023/A:1008206212145
Publication status	Published - May 1998
Externally published	Yes

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title = "Heteronuclear correlation experiments for the determination of one-bond coupling constants",

abstract = "Spin-state selective experiments, HSQC-α/β and CT-HMQC-α/β, are proposed for the simple and rapid measurement of scalar one-bond coupling constants in two-dimensional, 1H-detected 15N-1H or 13C-1H correlation experiments based on HSQC and HMQC schemes. Pairs of subspectra are obtained, containing either the high-field or the low-field component of the doublet representing the one-bond coupling constant. The subspectral editing procedure retains the full sensitivity of HSQC and HMQC spectra recorded without heteronuclear decoupling during data acquisition, with a spectral resolution similar to that of decoupled spectra.",

keywords = "E. coli arginine repressor, HMQC-α/β, HSQC-α/β, One-bond coupling constants",

author = "Patrik Andersson and Kerstin Nordstrand and Maria Sunnerhagen and Edvards Liepinsh and Ivars Turovskis and Gottfried Otting",

year = "1998",

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doi = "10.1023/A:1008206212145",

language = "English",

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pages = "445--450",

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TY - JOUR

T1 - Heteronuclear correlation experiments for the determination of one-bond coupling constants

AU - Andersson, Patrik

AU - Nordstrand, Kerstin

AU - Sunnerhagen, Maria

AU - Liepinsh, Edvards

AU - Turovskis, Ivars

AU - Otting, Gottfried

PY - 1998/5

Y1 - 1998/5

N2 - Spin-state selective experiments, HSQC-α/β and CT-HMQC-α/β, are proposed for the simple and rapid measurement of scalar one-bond coupling constants in two-dimensional, 1H-detected 15N-1H or 13C-1H correlation experiments based on HSQC and HMQC schemes. Pairs of subspectra are obtained, containing either the high-field or the low-field component of the doublet representing the one-bond coupling constant. The subspectral editing procedure retains the full sensitivity of HSQC and HMQC spectra recorded without heteronuclear decoupling during data acquisition, with a spectral resolution similar to that of decoupled spectra.

AB - Spin-state selective experiments, HSQC-α/β and CT-HMQC-α/β, are proposed for the simple and rapid measurement of scalar one-bond coupling constants in two-dimensional, 1H-detected 15N-1H or 13C-1H correlation experiments based on HSQC and HMQC schemes. Pairs of subspectra are obtained, containing either the high-field or the low-field component of the doublet representing the one-bond coupling constant. The subspectral editing procedure retains the full sensitivity of HSQC and HMQC spectra recorded without heteronuclear decoupling during data acquisition, with a spectral resolution similar to that of decoupled spectra.

KW - E. coli arginine repressor

KW - HMQC-α/β

KW - HSQC-α/β

KW - One-bond coupling constants

UR - https://www.scopus.com/pages/publications/0032062082

U2 - 10.1023/A:1008206212145

DO - 10.1023/A:1008206212145

M3 - Article

SN - 0925-2738

VL - 11

SP - 445

EP - 450

JO - Journal of Biomolecular NMR

JF - Journal of Biomolecular NMR

IS - 4

ER -

Heteronuclear correlation experiments for the determination of one-bond coupling constants

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