Hofmeister effects in biology: Effect of choline addition on the salt-induced super activity of horseradish peroxidase and its implication for salt resistance of plants

M. C. Pinna, P. Bauduin, D. Touraud, M. Monduzzi, B. W. Ninham, W. Kunz*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    72 Citations (Scopus)

    Abstract

    The effect of choline addition on the salt-induced super activity of horseradish peroxidase (HRP) is investigated. HRP is presented in the literature as an efficient H 2O 2 scavenger, and choline is the precursor of glycine betaine, a strong osmoprotectant molecule. Both the regulations of H 2O 2 and of osmoprotectant concentrations are implicated in plants in order to counteract salt-induced cell damage. For the oxidation of 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), sulfate anions were found to play a crucial role in the increase of HRP activity. This induced super activity can be strongly reduced by adding choline chloride. The phenomena provide an example of physicochemical Hofmeister effects playing a central regulatory role in an important biological system.

    Original languageEnglish
    Pages (from-to)16511-16514
    Number of pages4
    JournalJournal of Physical Chemistry B
    Volume109
    Issue number34
    DOIs
    Publication statusPublished - 1 Sept 2005

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