Abstract
The effect of choline addition on the salt-induced super activity of horseradish peroxidase (HRP) is investigated. HRP is presented in the literature as an efficient H 2O 2 scavenger, and choline is the precursor of glycine betaine, a strong osmoprotectant molecule. Both the regulations of H 2O 2 and of osmoprotectant concentrations are implicated in plants in order to counteract salt-induced cell damage. For the oxidation of 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), sulfate anions were found to play a crucial role in the increase of HRP activity. This induced super activity can be strongly reduced by adding choline chloride. The phenomena provide an example of physicochemical Hofmeister effects playing a central regulatory role in an important biological system.
Original language | English |
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Pages (from-to) | 16511-16514 |
Number of pages | 4 |
Journal | Journal of Physical Chemistry B |
Volume | 109 |
Issue number | 34 |
DOIs | |
Publication status | Published - 1 Sept 2005 |