Hofmeister effects in biology: Effect of choline addition on the salt-induced super activity of horseradish peroxidase and its implication for salt resistance of plants

M. C. Pinna; P. Bauduin; D. Touraud; M. Monduzzi; B. W. Ninham; W. Kunz

doi:10.1021/jp051897v

Hofmeister effects in biology: Effect of choline addition on the salt-induced super activity of horseradish peroxidase and its implication for salt resistance of plants

M. C. Pinna, P. Bauduin, D. Touraud, M. Monduzzi, B. W. Ninham, W. Kunz^*

^*Corresponding author for this work

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Abstract

The effect of choline addition on the salt-induced super activity of horseradish peroxidase (HRP) is investigated. HRP is presented in the literature as an efficient H ₂O ₂ scavenger, and choline is the precursor of glycine betaine, a strong osmoprotectant molecule. Both the regulations of H ₂O ₂ and of osmoprotectant concentrations are implicated in plants in order to counteract salt-induced cell damage. For the oxidation of 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), sulfate anions were found to play a crucial role in the increase of HRP activity. This induced super activity can be strongly reduced by adding choline chloride. The phenomena provide an example of physicochemical Hofmeister effects playing a central regulatory role in an important biological system.

Original language	English
Pages (from-to)	16511-16514
Number of pages	4
Journal	Journal of Physical Chemistry B
Volume	109
Issue number	34
DOIs	https://doi.org/10.1021/jp051897v
Publication status	Published - 1 Sept 2005

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title = "Hofmeister effects in biology: Effect of choline addition on the salt-induced super activity of horseradish peroxidase and its implication for salt resistance of plants",

abstract = "The effect of choline addition on the salt-induced super activity of horseradish peroxidase (HRP) is investigated. HRP is presented in the literature as an efficient H 2O 2 scavenger, and choline is the precursor of glycine betaine, a strong osmoprotectant molecule. Both the regulations of H 2O 2 and of osmoprotectant concentrations are implicated in plants in order to counteract salt-induced cell damage. For the oxidation of 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), sulfate anions were found to play a crucial role in the increase of HRP activity. This induced super activity can be strongly reduced by adding choline chloride. The phenomena provide an example of physicochemical Hofmeister effects playing a central regulatory role in an important biological system.",

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T2 - Effect of choline addition on the salt-induced super activity of horseradish peroxidase and its implication for salt resistance of plants

AU - Pinna, M. C.

AU - Bauduin, P.

AU - Touraud, D.

AU - Monduzzi, M.

AU - Ninham, B. W.

AU - Kunz, W.

PY - 2005/9/1

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AB - The effect of choline addition on the salt-induced super activity of horseradish peroxidase (HRP) is investigated. HRP is presented in the literature as an efficient H 2O 2 scavenger, and choline is the precursor of glycine betaine, a strong osmoprotectant molecule. Both the regulations of H 2O 2 and of osmoprotectant concentrations are implicated in plants in order to counteract salt-induced cell damage. For the oxidation of 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), sulfate anions were found to play a crucial role in the increase of HRP activity. This induced super activity can be strongly reduced by adding choline chloride. The phenomena provide an example of physicochemical Hofmeister effects playing a central regulatory role in an important biological system.

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Hofmeister effects in biology: Effect of choline addition on the salt-induced super activity of horseradish peroxidase and its implication for salt resistance of plants

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