Holographic microscope and its biological application

Xiangyu Quan; Daisuke Kato; Vincent Daria; Osamu Matoba; Hiroaki Wake

doi:10.1016/j.neures.2021.10.012

Holographic microscope and its biological application

Xiangyu Quan, Daisuke Kato, Vincent Daria, Osamu Matoba, Hiroaki Wake^*

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

8 Citations (Scopus)

Abstract

Holographic structured illumination combined with optogenetics enables patterned stimulation of neurons and glial cells in an intact living brain. Moreover, in vivo functional imaging of cellular activity with recent advanced microscope technologies allows for visualization of the cellular responses during learning, emotion and cognition. Integrating these techniques can be used to verify the link between cell function and behavior output. However, there are technical limitations to stimulate multiple cells with high spatial and temporal resolution with available techniques of optogenetic stimulation. Here, we summarized a two-photon microscope combined with holographic system to stimulate multiple cells with high spatial and temporal resolution for living mice and their biological application.

Original language	English
Pages (from-to)	57-64
Number of pages	8
Journal	Neuroscience Research
Volume	179
DOIs	https://doi.org/10.1016/j.neures.2021.10.012
Publication status	Published - Jun 2022

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10.1016/j.neures.2021.10.012

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title = "Holographic microscope and its biological application",

abstract = "Holographic structured illumination combined with optogenetics enables patterned stimulation of neurons and glial cells in an intact living brain. Moreover, in vivo functional imaging of cellular activity with recent advanced microscope technologies allows for visualization of the cellular responses during learning, emotion and cognition. Integrating these techniques can be used to verify the link between cell function and behavior output. However, there are technical limitations to stimulate multiple cells with high spatial and temporal resolution with available techniques of optogenetic stimulation. Here, we summarized a two-photon microscope combined with holographic system to stimulate multiple cells with high spatial and temporal resolution for living mice and their biological application.",

keywords = "holographic microscope, optogenetics, three-dimensional (3D) light patterns, two-photon microscope",

author = "Xiangyu Quan and Daisuke Kato and Vincent Daria and Osamu Matoba and Hiroaki Wake",

note = "Publisher Copyright: {\textcopyright} 2021 The Authors",

year = "2022",

month = jun,

doi = "10.1016/j.neures.2021.10.012",

language = "English",

volume = "179",

pages = "57--64",

journal = "Neuroscience Research",

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T1 - Holographic microscope and its biological application

AU - Quan, Xiangyu

AU - Kato, Daisuke

AU - Daria, Vincent

AU - Matoba, Osamu

AU - Wake, Hiroaki

PY - 2022/6

Y1 - 2022/6

N2 - Holographic structured illumination combined with optogenetics enables patterned stimulation of neurons and glial cells in an intact living brain. Moreover, in vivo functional imaging of cellular activity with recent advanced microscope technologies allows for visualization of the cellular responses during learning, emotion and cognition. Integrating these techniques can be used to verify the link between cell function and behavior output. However, there are technical limitations to stimulate multiple cells with high spatial and temporal resolution with available techniques of optogenetic stimulation. Here, we summarized a two-photon microscope combined with holographic system to stimulate multiple cells with high spatial and temporal resolution for living mice and their biological application.

AB - Holographic structured illumination combined with optogenetics enables patterned stimulation of neurons and glial cells in an intact living brain. Moreover, in vivo functional imaging of cellular activity with recent advanced microscope technologies allows for visualization of the cellular responses during learning, emotion and cognition. Integrating these techniques can be used to verify the link between cell function and behavior output. However, there are technical limitations to stimulate multiple cells with high spatial and temporal resolution with available techniques of optogenetic stimulation. Here, we summarized a two-photon microscope combined with holographic system to stimulate multiple cells with high spatial and temporal resolution for living mice and their biological application.

KW - holographic microscope

KW - optogenetics

KW - three-dimensional (3D) light patterns

KW - two-photon microscope

UR - http://www.scopus.com/inward/record.url?scp=85119303081&partnerID=8YFLogxK

U2 - 10.1016/j.neures.2021.10.012

DO - 10.1016/j.neures.2021.10.012

M3 - Review article

SN - 0168-0102

VL - 179

SP - 57

EP - 64

JO - Neuroscience Research

JF - Neuroscience Research

ER -

Holographic microscope and its biological application

Abstract

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