Host attachment, invasion, and stimulation of proinflammatory cytokines by campylobacter concisus and other non-campylobacter jejuni Campylobacter species

Si Ming Man, Nadeem O. Kaakoush, Steven T. Leach, Lily Nahidi, Hao K. Lu, Jennifer Norman, Andrew S. Day, Li Zhang, Hazel M. Mitchell

Research output: Contribution to journalArticlepeer-review

105 Citations (Scopus)

Abstract

Background: Campylobacter concisus and other non-Campylobacter jejuni Campylobacter species have been implicated in the initiation of gastrointestinal diseases. In the present study, we investigated the interaction between these bacteria and the human intestinal epithelium and immune cells. Methods: The ability of C. concisus, Campylobacter showae, Campylobacter hominis, and Bacteroides ureolyticus to invade epithelial cells was examined using scanning electron microscopy and gentamicin protection assays. Proinflammatory cytokines generated by epithelial and immune cells in response to these bacteria were determined by enzyme-linked immunosorbent assay. Ussing Chamber, immunofluorescent stain, and Western blot were used to further elucidate the impact of C. concisus on intestinal barrier integrity and functions. Results: Attachment of non-C. jejuni Campylobacter species to Caco-2 or HT-29 cells was mediated by flagellum-dependent and/or -independent processes. C. concisus was able to invade Caco-2 cells, generate a membraneruffling effect on the epithelial surface on entry, and damage epithelial barrier functions by preferential attachment to the cell-cell junctions. Proinflammatory cytokine profiles exhibited by epithelial cells, monocytes, and macrophages in response to C. concisus and other non-C. jejuni Campylobacter species were species and strain specific. Conclusions: These findings demonstrate that C. concisus and other non-C. jejuni Campylobacter species may play a role in initiating gastrointestinal diseases.

Original languageEnglish
Pages (from-to)1855-1865
Number of pages11
JournalJournal of Infectious Diseases
Volume202
Issue number12
DOIs
Publication statusPublished - 15 Dec 2010
Externally publishedYes

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