How plasma induced oxidation, oxygenation, and de-oxygenation influences viability of skin cells

Jun Seok Oh; Xanthe Strudwick; Robert D. Short; Kotaro Ogawa; Akimitsu Hatta; Hiroshi Furuta; Nishtha Gaur; Sung Ha Hong; Allison J. Cowin; Hideo Fukuhara; Keiji Inoue; Masafumi Ito; Christine Charles; Roderick W. Boswell; James W. Bradley; David B. Graves; Endre J. Szili

doi:10.1063/1.4967880

How plasma induced oxidation, oxygenation, and de-oxygenation influences viability of skin cells

Jun Seok Oh^*, Xanthe Strudwick, Robert D. Short, Kotaro Ogawa, Akimitsu Hatta, Hiroshi Furuta, Nishtha Gaur, Sung Ha Hong, Allison J. Cowin, Hideo Fukuhara, Keiji Inoue, Masafumi Ito, Christine Charles, Roderick W. Boswell, James W. Bradley, David B. Graves, Endre J. Szili

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

The effect of oxidation, oxygenation, and de-oxygenation arising from He gas jet and He plasma jet treatments on the viability of skin cells cultured in vitro has been investigated. He gas jet treatment de-oxygenated cell culture medium in a process referred to as "sparging." He plasma jet treatments oxidized, as well as oxygenated or de-oxygenated cell culture medium depending on the dissolved oxygen concentration at the time of treatment. He gas and plasma jets were shown to have beneficial or deleterious effects on skin cells depending on the concentration of dissolved oxygen and other oxidative molecules at the time of treatment. Different combinations of treatments with He gas and plasma jets can be used to modulate the concentrations of dissolved oxygen and other oxidative molecules to influence cell viability. This study highlights the importance of a priori knowledge of the concentration of dissolved oxygen at the time of plasma jet treatment, given the potential for significant impact on the biological or medical outcome. Monitoring and controlling the dynamic changes in dissolved oxygen is essential in order to develop effective strategies for the use of cold atmospheric plasma jets in biology and medicine.

Original language	English
Article number	203701
Journal	Applied Physics Letters
Volume	109
Issue number	20
DOIs	https://doi.org/10.1063/1.4967880
Publication status	Published - 14 Nov 2016

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Oh, J. S., Strudwick, X., Short, R. D., Ogawa, K., Hatta, A., Furuta, H., Gaur, N., Hong, S. H., Cowin, A. J., Fukuhara, H., Inoue, K., Ito, M., Charles, C., Boswell, R. W., Bradley, J. W., Graves, D. B., & Szili, E. J. (2016). How plasma induced oxidation, oxygenation, and de-oxygenation influences viability of skin cells. Applied Physics Letters, 109(20), Article 203701. https://doi.org/10.1063/1.4967880

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title = "How plasma induced oxidation, oxygenation, and de-oxygenation influences viability of skin cells",

abstract = "The effect of oxidation, oxygenation, and de-oxygenation arising from He gas jet and He plasma jet treatments on the viability of skin cells cultured in vitro has been investigated. He gas jet treatment de-oxygenated cell culture medium in a process referred to as {"}sparging.{"} He plasma jet treatments oxidized, as well as oxygenated or de-oxygenated cell culture medium depending on the dissolved oxygen concentration at the time of treatment. He gas and plasma jets were shown to have beneficial or deleterious effects on skin cells depending on the concentration of dissolved oxygen and other oxidative molecules at the time of treatment. Different combinations of treatments with He gas and plasma jets can be used to modulate the concentrations of dissolved oxygen and other oxidative molecules to influence cell viability. This study highlights the importance of a priori knowledge of the concentration of dissolved oxygen at the time of plasma jet treatment, given the potential for significant impact on the biological or medical outcome. Monitoring and controlling the dynamic changes in dissolved oxygen is essential in order to develop effective strategies for the use of cold atmospheric plasma jets in biology and medicine.",

author = "Oh, {Jun Seok} and Xanthe Strudwick and Short, {Robert D.} and Kotaro Ogawa and Akimitsu Hatta and Hiroshi Furuta and Nishtha Gaur and Hong, {Sung Ha} and Cowin, {Allison J.} and Hideo Fukuhara and Keiji Inoue and Masafumi Ito and Christine Charles and Boswell, {Roderick W.} and Bradley, {James W.} and Graves, {David B.} and Szili, {Endre J.}",

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year = "2016",

month = nov,

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doi = "10.1063/1.4967880",

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Oh, JS, Strudwick, X, Short, RD, Ogawa, K, Hatta, A, Furuta, H, Gaur, N, Hong, SH, Cowin, AJ, Fukuhara, H, Inoue, K, Ito, M, Charles, C , Boswell, RW, Bradley, JW, Graves, DB & Szili, EJ 2016, 'How plasma induced oxidation, oxygenation, and de-oxygenation influences viability of skin cells', Applied Physics Letters, vol. 109, no. 20, 203701. https://doi.org/10.1063/1.4967880

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T1 - How plasma induced oxidation, oxygenation, and de-oxygenation influences viability of skin cells

AU - Oh, Jun Seok

AU - Strudwick, Xanthe

AU - Short, Robert D.

AU - Ogawa, Kotaro

AU - Hatta, Akimitsu

AU - Furuta, Hiroshi

AU - Gaur, Nishtha

AU - Hong, Sung Ha

AU - Cowin, Allison J.

AU - Fukuhara, Hideo

AU - Inoue, Keiji

AU - Ito, Masafumi

AU - Charles, Christine

AU - Boswell, Roderick W.

AU - Bradley, James W.

AU - Graves, David B.

AU - Szili, Endre J.

PY - 2016/11/14

Y1 - 2016/11/14

N2 - The effect of oxidation, oxygenation, and de-oxygenation arising from He gas jet and He plasma jet treatments on the viability of skin cells cultured in vitro has been investigated. He gas jet treatment de-oxygenated cell culture medium in a process referred to as "sparging." He plasma jet treatments oxidized, as well as oxygenated or de-oxygenated cell culture medium depending on the dissolved oxygen concentration at the time of treatment. He gas and plasma jets were shown to have beneficial or deleterious effects on skin cells depending on the concentration of dissolved oxygen and other oxidative molecules at the time of treatment. Different combinations of treatments with He gas and plasma jets can be used to modulate the concentrations of dissolved oxygen and other oxidative molecules to influence cell viability. This study highlights the importance of a priori knowledge of the concentration of dissolved oxygen at the time of plasma jet treatment, given the potential for significant impact on the biological or medical outcome. Monitoring and controlling the dynamic changes in dissolved oxygen is essential in order to develop effective strategies for the use of cold atmospheric plasma jets in biology and medicine.

AB - The effect of oxidation, oxygenation, and de-oxygenation arising from He gas jet and He plasma jet treatments on the viability of skin cells cultured in vitro has been investigated. He gas jet treatment de-oxygenated cell culture medium in a process referred to as "sparging." He plasma jet treatments oxidized, as well as oxygenated or de-oxygenated cell culture medium depending on the dissolved oxygen concentration at the time of treatment. He gas and plasma jets were shown to have beneficial or deleterious effects on skin cells depending on the concentration of dissolved oxygen and other oxidative molecules at the time of treatment. Different combinations of treatments with He gas and plasma jets can be used to modulate the concentrations of dissolved oxygen and other oxidative molecules to influence cell viability. This study highlights the importance of a priori knowledge of the concentration of dissolved oxygen at the time of plasma jet treatment, given the potential for significant impact on the biological or medical outcome. Monitoring and controlling the dynamic changes in dissolved oxygen is essential in order to develop effective strategies for the use of cold atmospheric plasma jets in biology and medicine.

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VL - 109

JO - Applied Physics Letters

JF - Applied Physics Letters

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M1 - 203701

ER -

How plasma induced oxidation, oxygenation, and de-oxygenation influences viability of skin cells

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