Identification and characterization of GSTT3, a third murine Theta class glutathione transferase

Marjorie Coggan; Jack U. Flanagan; Michael W. Parker; Vanicha Vichai; William R. Pearson; Philip G. Board

doi:10.1042/BJ20011878

Identification and characterization of GSTT3, a third murine Theta class glutathione transferase

Marjorie Coggan, Jack U. Flanagan, Michael W. Parker, Vanicha Vichai, William R. Pearson, Philip G. Board^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

A novel Theta class glutathione transferase (GST) isoenzyme from mouse termed mGSTT3 has been identified by analysis of the expressed sequence tag database. The gene encoding mGSTT3 is clustered with the mGSTT1 and mGSTT2 genes on chromosome 10 and has an exon/intron structure that is similar to that of the other Theta class genes. mGSTT3 is expressed strongly in the liver and to a decreasing extent in the kidney and testis. Recombinant mGSTT3-3 expressed in Escherichia coli had a substrate-specificity profile that differed significantly from that of GSTT1-1 and GSTT2-2 isoenzymes. A molecular model of mGSTT3 suggested that, in comparison with GSTT2, a decrease in volume of the hydrophobic substrate-binding site and the loss of the sulphate-binding pocket prevents its use of the GSTT2 substrate 1-menaphthyl sulphate.

Original language	English
Pages (from-to)	323-332
Number of pages	10
Journal	Biochemical Journal
Volume	366
Issue number	1
DOIs	https://doi.org/10.1042/BJ20011878
Publication status	Published - 15 Aug 2002

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title = "Identification and characterization of GSTT3, a third murine Theta class glutathione transferase",

abstract = "A novel Theta class glutathione transferase (GST) isoenzyme from mouse termed mGSTT3 has been identified by analysis of the expressed sequence tag database. The gene encoding mGSTT3 is clustered with the mGSTT1 and mGSTT2 genes on chromosome 10 and has an exon/intron structure that is similar to that of the other Theta class genes. mGSTT3 is expressed strongly in the liver and to a decreasing extent in the kidney and testis. Recombinant mGSTT3-3 expressed in Escherichia coli had a substrate-specificity profile that differed significantly from that of GSTT1-1 and GSTT2-2 isoenzymes. A molecular model of mGSTT3 suggested that, in comparison with GSTT2, a decrease in volume of the hydrophobic substrate-binding site and the loss of the sulphate-binding pocket prevents its use of the GSTT2 substrate 1-menaphthyl sulphate.",

keywords = "Database analysis, Enzyme, Glutathione transferase gene",

author = "Marjorie Coggan and Flanagan, {Jack U.} and Parker, {Michael W.} and Vanicha Vichai and Pearson, {William R.} and Board, {Philip G.}",

year = "2002",

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T1 - Identification and characterization of GSTT3, a third murine Theta class glutathione transferase

AU - Coggan, Marjorie

AU - Flanagan, Jack U.

AU - Parker, Michael W.

AU - Vichai, Vanicha

AU - Pearson, William R.

AU - Board, Philip G.

PY - 2002/8/15

Y1 - 2002/8/15

N2 - A novel Theta class glutathione transferase (GST) isoenzyme from mouse termed mGSTT3 has been identified by analysis of the expressed sequence tag database. The gene encoding mGSTT3 is clustered with the mGSTT1 and mGSTT2 genes on chromosome 10 and has an exon/intron structure that is similar to that of the other Theta class genes. mGSTT3 is expressed strongly in the liver and to a decreasing extent in the kidney and testis. Recombinant mGSTT3-3 expressed in Escherichia coli had a substrate-specificity profile that differed significantly from that of GSTT1-1 and GSTT2-2 isoenzymes. A molecular model of mGSTT3 suggested that, in comparison with GSTT2, a decrease in volume of the hydrophobic substrate-binding site and the loss of the sulphate-binding pocket prevents its use of the GSTT2 substrate 1-menaphthyl sulphate.

AB - A novel Theta class glutathione transferase (GST) isoenzyme from mouse termed mGSTT3 has been identified by analysis of the expressed sequence tag database. The gene encoding mGSTT3 is clustered with the mGSTT1 and mGSTT2 genes on chromosome 10 and has an exon/intron structure that is similar to that of the other Theta class genes. mGSTT3 is expressed strongly in the liver and to a decreasing extent in the kidney and testis. Recombinant mGSTT3-3 expressed in Escherichia coli had a substrate-specificity profile that differed significantly from that of GSTT1-1 and GSTT2-2 isoenzymes. A molecular model of mGSTT3 suggested that, in comparison with GSTT2, a decrease in volume of the hydrophobic substrate-binding site and the loss of the sulphate-binding pocket prevents its use of the GSTT2 substrate 1-menaphthyl sulphate.

KW - Database analysis

KW - Enzyme

KW - Glutathione transferase gene

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DO - 10.1042/BJ20011878

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VL - 366

SP - 323

EP - 332

JO - Biochemical Journal

JF - Biochemical Journal

IS - 1

ER -

Identification and characterization of GSTT3, a third murine Theta class glutathione transferase

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