TY - JOUR
T1 - Identification of cryptic sex chromosomes and isolation of X- and Y-borne genes.
AU - Waters, Paul D.
AU - Marshall Graves, Jennifer A.
AU - Thompson, Katherine
AU - Sankovic, Natasha
AU - Ezaz, Tariq
PY - 2008
Y1 - 2008
N2 - Comparative molecular cytogenetics provides a powerful tool for deciphering the evolutionary history of vertebrate sex chromosomes. We have adapted cell culture and molecular cytogenetic techniques to study the sex chromosomes of many exotic mammals, birds, and reptiles. Here we describe differential chromosome banding and staining techniques that distinguish sex chromosomes in species with no morphologically distinct XY or ZW chromosome pairs. We describe a method to isolate, identify, and map genomic BAC clones from the Y chromosome, and we also identify strategies for isolating candidate sex chromosome genes.
AB - Comparative molecular cytogenetics provides a powerful tool for deciphering the evolutionary history of vertebrate sex chromosomes. We have adapted cell culture and molecular cytogenetic techniques to study the sex chromosomes of many exotic mammals, birds, and reptiles. Here we describe differential chromosome banding and staining techniques that distinguish sex chromosomes in species with no morphologically distinct XY or ZW chromosome pairs. We describe a method to isolate, identify, and map genomic BAC clones from the Y chromosome, and we also identify strategies for isolating candidate sex chromosome genes.
UR - http://www.scopus.com/inward/record.url?scp=48849086020&partnerID=8YFLogxK
U2 - 10.1007/978-1-59745-581-7_15
DO - 10.1007/978-1-59745-581-7_15
M3 - Article
C2 - 18629671
AN - SCOPUS:48849086020
SN - 1064-3745
VL - 422
SP - 239
EP - 251
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
ER -