Immune response to rotavirus VP4 expressed in an attenuated strain of Shigella flexneri

Adèle L. Loy; Gwen Allison; Carlos F. Arias; Naresh K. Verma

doi:10.1016/S0928-8244(99)00098-X

Immune response to rotavirus VP4 expressed in an attenuated strain of Shigella flexneri

Adèle L. Loy, Gwen Allison, Carlos F. Arias, Naresh K. Verma^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

An attenuated strain of Shigella flexneri was utilised to express viral protein (VP) 4 of rotavirus and the immunogenicity of the recombinant constructs was studied in BALB/c mice. VP4 was expressed as a fusion with maltose binding protein (MBP) in both the cytoplasm and periplasm, with a much higher level of expression occurring in the former. While all constructs induced a Shigella-specific response in mice, only the construct expressing MBP-VP4 in the cytoplasm of Shigella stimulated an immune response specific to rotavirus. This study demonstrates that Shigella can be used to deliver rotavirus antigens and induces an immune response directed towards both rotavirus and Shigella. Copyright (C) 1999 Federation of European Microbiological Societies.

Original language	English
Pages (from-to)	283-288
Number of pages	6
Journal	FEMS Immunology and Medical Microbiology
Volume	25
Issue number	3
DOIs	https://doi.org/10.1016/S0928-8244(99)00098-X
Publication status	Published - 15 Aug 1999

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title = "Immune response to rotavirus VP4 expressed in an attenuated strain of Shigella flexneri",

abstract = "An attenuated strain of Shigella flexneri was utilised to express viral protein (VP) 4 of rotavirus and the immunogenicity of the recombinant constructs was studied in BALB/c mice. VP4 was expressed as a fusion with maltose binding protein (MBP) in both the cytoplasm and periplasm, with a much higher level of expression occurring in the former. While all constructs induced a Shigella-specific response in mice, only the construct expressing MBP-VP4 in the cytoplasm of Shigella stimulated an immune response specific to rotavirus. This study demonstrates that Shigella can be used to deliver rotavirus antigens and induces an immune response directed towards both rotavirus and Shigella. Copyright (C) 1999 Federation of European Microbiological Societies.",

keywords = "Fusion protein, Immunogenicity, Rotavirus, Shigella flexneri, Viral protein 4",

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AU - Loy, Adèle L.

AU - Allison, Gwen

AU - Arias, Carlos F.

AU - Verma, Naresh K.

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AB - An attenuated strain of Shigella flexneri was utilised to express viral protein (VP) 4 of rotavirus and the immunogenicity of the recombinant constructs was studied in BALB/c mice. VP4 was expressed as a fusion with maltose binding protein (MBP) in both the cytoplasm and periplasm, with a much higher level of expression occurring in the former. While all constructs induced a Shigella-specific response in mice, only the construct expressing MBP-VP4 in the cytoplasm of Shigella stimulated an immune response specific to rotavirus. This study demonstrates that Shigella can be used to deliver rotavirus antigens and induces an immune response directed towards both rotavirus and Shigella. Copyright (C) 1999 Federation of European Microbiological Societies.

KW - Fusion protein

KW - Immunogenicity

KW - Rotavirus

KW - Shigella flexneri

KW - Viral protein 4

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JO - FEMS Immunology and Medical Microbiology

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Immune response to rotavirus VP4 expressed in an attenuated strain of Shigella flexneri

Abstract

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