Immunology by numbers: Quantitation of antigen presentation completes the quantitative milieu of systems immunology!

Anthony W. Purcell; Nathan P. Croft; David C. Tscharke

doi:10.1016/j.coi.2016.03.007

Immunology by numbers: Quantitation of antigen presentation completes the quantitative milieu of systems immunology!

Anthony W. Purcell^*, Nathan P. Croft, David C. Tscharke

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

24 Citations (Scopus)

Abstract

We review approaches to quantitate antigen presentation using a variety of biological and biochemical readouts and highlight the emerging role of mass spectrometry (MS) in defining and quantifying MHC-bound peptides presented at the cell surface. The combination of high mass accuracy in the determination of the molecular weight of the intact peptide of interest and its signature pattern of fragmentation during tandem MS provide an unambiguous and definitive identification. This is in contrast to the potential receptor cross-reactivity towards closely related peptides and variable dose responsiveness seen in biological readouts. In addition, we gaze into the not too distant future where big data approaches in MS can be accommodated to quantify whole immunopeptidomes both in vitro and in vivo.

Original language	English
Pages (from-to)	88-95
Number of pages	8
Journal	Current Opinion in Immunology
Volume	40
DOIs	https://doi.org/10.1016/j.coi.2016.03.007
Publication status	Published - 1 Jun 2016

Access to Document

10.1016/j.coi.2016.03.007

Cite this

@article{9fb8b2e3ffe441a78f648a077eef254e,

title = "Immunology by numbers: Quantitation of antigen presentation completes the quantitative milieu of systems immunology!",

abstract = "We review approaches to quantitate antigen presentation using a variety of biological and biochemical readouts and highlight the emerging role of mass spectrometry (MS) in defining and quantifying MHC-bound peptides presented at the cell surface. The combination of high mass accuracy in the determination of the molecular weight of the intact peptide of interest and its signature pattern of fragmentation during tandem MS provide an unambiguous and definitive identification. This is in contrast to the potential receptor cross-reactivity towards closely related peptides and variable dose responsiveness seen in biological readouts. In addition, we gaze into the not too distant future where big data approaches in MS can be accommodated to quantify whole immunopeptidomes both in vitro and in vivo.",

author = "Purcell, {Anthony W.} and Croft, {Nathan P.} and Tscharke, {David C.}",

note = "Publisher Copyright: {\textcopyright} 2016 .",

year = "2016",

month = jun,

day = "1",

doi = "10.1016/j.coi.2016.03.007",

language = "English",

volume = "40",

pages = "88--95",

journal = "Current Opinion in Immunology",

issn = "0952-7915",

publisher = "Elsevier Ltd.",

}

TY - JOUR

T1 - Immunology by numbers

T2 - Quantitation of antigen presentation completes the quantitative milieu of systems immunology!

AU - Purcell, Anthony W.

AU - Croft, Nathan P.

AU - Tscharke, David C.

PY - 2016/6/1

Y1 - 2016/6/1

N2 - We review approaches to quantitate antigen presentation using a variety of biological and biochemical readouts and highlight the emerging role of mass spectrometry (MS) in defining and quantifying MHC-bound peptides presented at the cell surface. The combination of high mass accuracy in the determination of the molecular weight of the intact peptide of interest and its signature pattern of fragmentation during tandem MS provide an unambiguous and definitive identification. This is in contrast to the potential receptor cross-reactivity towards closely related peptides and variable dose responsiveness seen in biological readouts. In addition, we gaze into the not too distant future where big data approaches in MS can be accommodated to quantify whole immunopeptidomes both in vitro and in vivo.

AB - We review approaches to quantitate antigen presentation using a variety of biological and biochemical readouts and highlight the emerging role of mass spectrometry (MS) in defining and quantifying MHC-bound peptides presented at the cell surface. The combination of high mass accuracy in the determination of the molecular weight of the intact peptide of interest and its signature pattern of fragmentation during tandem MS provide an unambiguous and definitive identification. This is in contrast to the potential receptor cross-reactivity towards closely related peptides and variable dose responsiveness seen in biological readouts. In addition, we gaze into the not too distant future where big data approaches in MS can be accommodated to quantify whole immunopeptidomes both in vitro and in vivo.

UR - http://www.scopus.com/inward/record.url?scp=84962348913&partnerID=8YFLogxK

U2 - 10.1016/j.coi.2016.03.007

DO - 10.1016/j.coi.2016.03.007

M3 - Review article

SN - 0952-7915

VL - 40

SP - 88

EP - 95

JO - Current Opinion in Immunology

JF - Current Opinion in Immunology

ER -

Immunology by numbers: Quantitation of antigen presentation completes the quantitative milieu of systems immunology!

Abstract

Access to Document

Other files and links

Fingerprint

Cite this