Immunology by numbers: Quantitation of antigen presentation completes the quantitative milieu of systems immunology!

Anthony W. Purcell*, Nathan P. Croft, David C. Tscharke

*Corresponding author for this work

    Research output: Contribution to journalReview articlepeer-review

    22 Citations (Scopus)

    Abstract

    We review approaches to quantitate antigen presentation using a variety of biological and biochemical readouts and highlight the emerging role of mass spectrometry (MS) in defining and quantifying MHC-bound peptides presented at the cell surface. The combination of high mass accuracy in the determination of the molecular weight of the intact peptide of interest and its signature pattern of fragmentation during tandem MS provide an unambiguous and definitive identification. This is in contrast to the potential receptor cross-reactivity towards closely related peptides and variable dose responsiveness seen in biological readouts. In addition, we gaze into the not too distant future where big data approaches in MS can be accommodated to quantify whole immunopeptidomes both in vitro and in vivo.

    Original languageEnglish
    Pages (from-to)88-95
    Number of pages8
    JournalCurrent Opinion in Immunology
    Volume40
    DOIs
    Publication statusPublished - 1 Jun 2016

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