In vitro culturing Plasmodium falciparum erythrocytic stages

Alexander Maier, Melanie Rug

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Abstract

    The in vitro cultivation of Plasmodium falciparum is absolutely essential for the molecular dissection of parasite biology and still poses several challenges. The dependence on, and interaction with host red blood cells, the tightly regulated stage-specific expression of proteins, and the parasite peculiar demands on nutrients and gaseous environments are only a few aspects that need to be addressed to successfully cultivate P. falciparum in vitro. In this chapter, we present techniques for normal maintenance of the erythrocytic stages of P. falciparum cultures, their synchronization and the generation of clonal cell lines.
    Original languageEnglish
    Title of host publicationMalaria: Methods and Protocols
    EditorsRobert Menard
    Place of PublicationUSA
    PublisherHumana Press
    Pages3-15pp
    Volume923
    Edition2nd
    ISBN (Print)9781627030250
    DOIs
    Publication statusPublished - 2013

    Publication series

    Name
    Volume923

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