In vitro culturing plasmodium falciparum erythrocytic stages

Alexander G. Maier, Melanie Rug

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

20 Citations (Scopus)

Abstract

The in vitro cultivation of Plasmodium falciparum is absolutely essential for the molecular dissection of parasite biology and still poses several challenges. The dependence on, and interaction with host red blood cells, the tightly regulated stage-specific expression of proteins, and the parasite peculiar demands on nutrients and gaseous environments are only a few aspects that need to be addressed to successfully cultivate P. falciparum in vitro. In this chapter, we present techniques for normal maintenance of the erythrocytic stages of P. falciparum cultures, their synchronization and the generation of clonal cell lines.

Original languageEnglish
Title of host publicationMalaria
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages3-15
Number of pages13
ISBN (Print)9781627030250
DOIs
Publication statusPublished - 2013

Publication series

NameMethods in Molecular Biology
Volume923
ISSN (Print)1064-3745

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