IN VITRO DIRECTED EVOLUTION OF ENZYMES EXPRESSED BY E COLI IN MICROTITER PLATES

Bradley Stevenson; Sylvia Yip; David Ollis

doi:10.1007/978-1-62703-293-3_18

IN VITRO DIRECTED EVOLUTION OF ENZYMES EXPRESSED BY E COLI IN MICROTITER PLATES

Bradley Stevenson, Sylvia Yip, David Ollis

Research output: Contribution to journal › Article › peer-review

Abstract

A method is described for using 96-well plates to prepare libraries of Escherichia coli cultures for screening a library of gene variants. This approach bypasses colony-picking to allow standard molecular biology laboratories to carry out directed evoluti

Original language	English
Pages (from-to)	237-249
Journal	Methods in Molecular Biology
Volume	978
DOIs	https://doi.org/10.1007/978-1-62703-293-3_18
Publication status	Published - 2013

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title = "IN VITRO DIRECTED EVOLUTION OF ENZYMES EXPRESSED BY E COLI IN MICROTITER PLATES",

abstract = "A method is described for using 96-well plates to prepare libraries of Escherichia coli cultures for screening a library of gene variants. This approach bypasses colony-picking to allow standard molecular biology laboratories to carry out directed evoluti",

author = "Bradley Stevenson and Sylvia Yip and David Ollis",

year = "2013",

doi = "10.1007/978-1-62703-293-3\_18",

language = "English",

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pages = "237--249",

journal = "Methods in Molecular Biology",

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AU - Stevenson, Bradley

AU - Yip, Sylvia

AU - Ollis, David

PY - 2013

Y1 - 2013

N2 - A method is described for using 96-well plates to prepare libraries of Escherichia coli cultures for screening a library of gene variants. This approach bypasses colony-picking to allow standard molecular biology laboratories to carry out directed evoluti

AB - A method is described for using 96-well plates to prepare libraries of Escherichia coli cultures for screening a library of gene variants. This approach bypasses colony-picking to allow standard molecular biology laboratories to carry out directed evoluti

UR - https://www.scopus.com/pages/publications/84882971966

U2 - 10.1007/978-1-62703-293-3_18

DO - 10.1007/978-1-62703-293-3_18

M3 - Article

VL - 978

SP - 237

EP - 249

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

IN VITRO DIRECTED EVOLUTION OF ENZYMES EXPRESSED BY E COLI IN MICROTITER PLATES

Abstract

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