Incorporation of chlorinated analogues of aliphatic amino acids during cell-free protein synthesis

Dannon J. Stigers; Zachary I. Watts; James E. Hennessy; Hye Kyung Kim; Romeo Martini; Matthew C. Taylor; Kiyoshi Ozawa; Jeffrey W. Keillor; Nicholas E. Dixon; Christopher J. Easton

doi:10.1039/c0cc02879g

Incorporation of chlorinated analogues of aliphatic amino acids during cell-free protein synthesis

Dannon J. Stigers, Zachary I. Watts, James E. Hennessy, Hye Kyung Kim, Romeo Martini, Matthew C. Taylor, Kiyoshi Ozawa, Jeffrey W. Keillor, Nicholas E. Dixon, Christopher J. Easton^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

3-Chloro-Abu and 4-chloro-Nva are biosynthetically incorporated into E. coli peptidyl-Pro cis-trans isomerase B, as substitutes for Val and Leu, respectively. The extent of incorporation is up to ∼90%, and substituted protein is catalytically active. By contrast, 4-chloro-Val is not an effective replacement for Ile.

Original language	English
Pages (from-to)	1839-1841
Number of pages	3
Journal	Chemical Communications
Volume	47
Issue number	6
DOIs	https://doi.org/10.1039/c0cc02879g
Publication status	Published - 27 Jan 2011

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title = "Incorporation of chlorinated analogues of aliphatic amino acids during cell-free protein synthesis",

abstract = "3-Chloro-Abu and 4-chloro-Nva are biosynthetically incorporated into E. coli peptidyl-Pro cis-trans isomerase B, as substitutes for Val and Leu, respectively. The extent of incorporation is up to ∼90%, and substituted protein is catalytically active. By contrast, 4-chloro-Val is not an effective replacement for Ile.",

author = "Stigers, {Dannon J.} and Watts, {Zachary I.} and Hennessy, {James E.} and Kim, {Hye Kyung} and Romeo Martini and Taylor, {Matthew C.} and Kiyoshi Ozawa and Keillor, {Jeffrey W.} and Dixon, {Nicholas E.} and Easton, {Christopher J.}",

year = "2011",

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doi = "10.1039/c0cc02879g",

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T1 - Incorporation of chlorinated analogues of aliphatic amino acids during cell-free protein synthesis

AU - Stigers, Dannon J.

AU - Watts, Zachary I.

AU - Hennessy, James E.

AU - Kim, Hye Kyung

AU - Martini, Romeo

AU - Taylor, Matthew C.

AU - Ozawa, Kiyoshi

AU - Keillor, Jeffrey W.

AU - Dixon, Nicholas E.

AU - Easton, Christopher J.

PY - 2011/1/27

Y1 - 2011/1/27

N2 - 3-Chloro-Abu and 4-chloro-Nva are biosynthetically incorporated into E. coli peptidyl-Pro cis-trans isomerase B, as substitutes for Val and Leu, respectively. The extent of incorporation is up to ∼90%, and substituted protein is catalytically active. By contrast, 4-chloro-Val is not an effective replacement for Ile.

AB - 3-Chloro-Abu and 4-chloro-Nva are biosynthetically incorporated into E. coli peptidyl-Pro cis-trans isomerase B, as substitutes for Val and Leu, respectively. The extent of incorporation is up to ∼90%, and substituted protein is catalytically active. By contrast, 4-chloro-Val is not an effective replacement for Ile.

UR - http://www.scopus.com/inward/record.url?scp=79251527551&partnerID=8YFLogxK

U2 - 10.1039/c0cc02879g

DO - 10.1039/c0cc02879g

M3 - Article

SN - 1359-7345

VL - 47

SP - 1839

EP - 1841

JO - Chemical Communications

JF - Chemical Communications

IS - 6

ER -

Incorporation of chlorinated analogues of aliphatic amino acids during cell-free protein synthesis

Abstract

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