TY - JOUR
T1 - Insight into the structural organization of the omega leader of TMV RNA
T2 - The role of various regions of the sequence in the formation of a compact structure of the omega RNA
AU - Agalarov, Sultan C.
AU - Sogorin, Evgeny A.
AU - Shirokikh, Nikolay E.
AU - Spirin, Alexander S.
PY - 2011/1/7
Y1 - 2011/1/7
N2 - The 5′-untranslated sequence of tobacco mosaic virus RNA - the so called omega leader - is a well-known translational enhancer. The structure of the omega RNA has unusual features. Despite the absence of extensive secondary structure of the Watson-Crick type, the omega RNA possesses a stable compact conformation. The central part of the omega sequence contains many CAA repeats and is flanked by U-rich regions. In this work we synthesized the polyribonucleotides containing modified omega sequences, and studied them using analytical ultracentrifugation and thermal melting techniques. It was demonstrated that changes made in both the central and the 3′-proximal part of the sequence led to a strong destabilization of the omega RNA structure. We conclude that the regular (CAA). n core region and the 3′-proximal AU-rich region of the omega RNA interact with each other and contribute together to the formation of a stable tertiary structure.
AB - The 5′-untranslated sequence of tobacco mosaic virus RNA - the so called omega leader - is a well-known translational enhancer. The structure of the omega RNA has unusual features. Despite the absence of extensive secondary structure of the Watson-Crick type, the omega RNA possesses a stable compact conformation. The central part of the omega sequence contains many CAA repeats and is flanked by U-rich regions. In this work we synthesized the polyribonucleotides containing modified omega sequences, and studied them using analytical ultracentrifugation and thermal melting techniques. It was demonstrated that changes made in both the central and the 3′-proximal part of the sequence led to a strong destabilization of the omega RNA structure. We conclude that the regular (CAA). n core region and the 3′-proximal AU-rich region of the omega RNA interact with each other and contribute together to the formation of a stable tertiary structure.
KW - Omega sequence
KW - Sedimentation analysis
KW - Temperature melting
KW - Tobacco mosaic virus (TMV) RNA
KW - Translational enhancers
UR - http://www.scopus.com/inward/record.url?scp=78650885500&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2010.11.102
DO - 10.1016/j.bbrc.2010.11.102
M3 - Article
SN - 0006-291X
VL - 404
SP - 250
EP - 253
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -