Insights into RNA Biology from an Atlas of Mammalian mRNA-Binding Proteins

Alfredo Castello; Bernd Fischer; Katrin Eichelbaum; Rastislav Horos; Benedikt M. Beckmann; Claudia Strein; Norman E. Davey; David T. Humphreys; Thomas Preiss; Lars M. Steinmetz; Jeroen Krijgsveld; Matthias W. Hentze

doi:10.1016/j.cell.2012.04.031

Insights into RNA Biology from an Atlas of Mammalian mRNA-Binding Proteins

Alfredo Castello, Bernd Fischer, Katrin Eichelbaum, Rastislav Horos, Benedikt M. Beckmann, Claudia Strein, Norman E. Davey, David T. Humphreys, Thomas Preiss, Lars M. Steinmetz, Jeroen Krijgsveld^*, Matthias W. Hentze

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1597 Citations (Scopus)

Abstract

RNA-binding proteins (RBPs) determine RNA fate from synthesis to decay. Employing two complementary protocols for covalent UV crosslinking of RBPs to RNA, we describe a systematic, unbiased, and comprehensive approach, termed "interactome capture," to define the mRNA interactome of proliferating human HeLa cells. We identify 860 proteins that qualify as RBPs by biochemical and statistical criteria, adding more than 300 RBPs to those previously known and shedding light on RBPs in disease, RNA-binding enzymes of intermediary metabolism, RNA-binding kinases, and RNA-binding architectures. Unexpectedly, we find that many proteins of the HeLa mRNA interactome are highly intrinsically disordered and enriched in short repetitive amino acid motifs. Interactome capture is broadly applicable to study mRNA interactome composition and dynamics in varied biological settings.

Original language	English
Pages (from-to)	1393-1406
Number of pages	14
Journal	Cell
Volume	149
Issue number	6
DOIs	https://doi.org/10.1016/j.cell.2012.04.031
Publication status	Published - 8 Jun 2012

Access to Document

10.1016/j.cell.2012.04.031

Cite this

@article{9ec0b6d41ed44af384897364d8d44e7e,

title = "Insights into RNA Biology from an Atlas of Mammalian mRNA-Binding Proteins",

abstract = "RNA-binding proteins (RBPs) determine RNA fate from synthesis to decay. Employing two complementary protocols for covalent UV crosslinking of RBPs to RNA, we describe a systematic, unbiased, and comprehensive approach, termed {"}interactome capture,{"} to define the mRNA interactome of proliferating human HeLa cells. We identify 860 proteins that qualify as RBPs by biochemical and statistical criteria, adding more than 300 RBPs to those previously known and shedding light on RBPs in disease, RNA-binding enzymes of intermediary metabolism, RNA-binding kinases, and RNA-binding architectures. Unexpectedly, we find that many proteins of the HeLa mRNA interactome are highly intrinsically disordered and enriched in short repetitive amino acid motifs. Interactome capture is broadly applicable to study mRNA interactome composition and dynamics in varied biological settings.",

author = "Alfredo Castello and Bernd Fischer and Katrin Eichelbaum and Rastislav Horos and Beckmann, {Benedikt M.} and Claudia Strein and Davey, {Norman E.} and Humphreys, {David T.} and Thomas Preiss and Steinmetz, {Lars M.} and Jeroen Krijgsveld and Hentze, {Matthias W.}",

year = "2012",

month = jun,

day = "8",

doi = "10.1016/j.cell.2012.04.031",

language = "English",

volume = "149",

pages = "1393--1406",

journal = "Cell",

issn = "0092-8674",

publisher = "Elsevier B.V.",

number = "6",

}

TY - JOUR

T1 - Insights into RNA Biology from an Atlas of Mammalian mRNA-Binding Proteins

AU - Castello, Alfredo

AU - Fischer, Bernd

AU - Eichelbaum, Katrin

AU - Horos, Rastislav

AU - Beckmann, Benedikt M.

AU - Strein, Claudia

AU - Davey, Norman E.

AU - Humphreys, David T.

AU - Preiss, Thomas

AU - Steinmetz, Lars M.

AU - Krijgsveld, Jeroen

AU - Hentze, Matthias W.

PY - 2012/6/8

Y1 - 2012/6/8

N2 - RNA-binding proteins (RBPs) determine RNA fate from synthesis to decay. Employing two complementary protocols for covalent UV crosslinking of RBPs to RNA, we describe a systematic, unbiased, and comprehensive approach, termed "interactome capture," to define the mRNA interactome of proliferating human HeLa cells. We identify 860 proteins that qualify as RBPs by biochemical and statistical criteria, adding more than 300 RBPs to those previously known and shedding light on RBPs in disease, RNA-binding enzymes of intermediary metabolism, RNA-binding kinases, and RNA-binding architectures. Unexpectedly, we find that many proteins of the HeLa mRNA interactome are highly intrinsically disordered and enriched in short repetitive amino acid motifs. Interactome capture is broadly applicable to study mRNA interactome composition and dynamics in varied biological settings.

AB - RNA-binding proteins (RBPs) determine RNA fate from synthesis to decay. Employing two complementary protocols for covalent UV crosslinking of RBPs to RNA, we describe a systematic, unbiased, and comprehensive approach, termed "interactome capture," to define the mRNA interactome of proliferating human HeLa cells. We identify 860 proteins that qualify as RBPs by biochemical and statistical criteria, adding more than 300 RBPs to those previously known and shedding light on RBPs in disease, RNA-binding enzymes of intermediary metabolism, RNA-binding kinases, and RNA-binding architectures. Unexpectedly, we find that many proteins of the HeLa mRNA interactome are highly intrinsically disordered and enriched in short repetitive amino acid motifs. Interactome capture is broadly applicable to study mRNA interactome composition and dynamics in varied biological settings.

UR - http://www.scopus.com/inward/record.url?scp=84861969926&partnerID=8YFLogxK

U2 - 10.1016/j.cell.2012.04.031

DO - 10.1016/j.cell.2012.04.031

M3 - Article

SN - 0092-8674

VL - 149

SP - 1393

EP - 1406

JO - Cell

JF - Cell

IS - 6

ER -

Insights into RNA Biology from an Atlas of Mammalian mRNA-Binding Proteins

Abstract

Access to Document

Other files and links

Fingerprint

Cite this