TY - GEN
T1 - Intensity isotherms and distributions on oligonucleotide microarrays
AU - Burden, C. J.
PY - 2009
Y1 - 2009
N2 - We describe a physico-chemical model relating measured fluorescence intensities on oligonucleotide microarrays to the underlying specific target concentration in the hybridised solution via a hyperbolic isotherm response function. The model includes various chemical reactions occurring at the microarray surface and in bulk solution during hybridisation, including specific and non-specific hybridisation, and also the effects of probe-target dissociation during the post hybridisation washing phase. We analyse the distribution of fluorescence intensities for a complete microarray in the light of this physicochemical model. Our results indicate that the majority of signals in a typical microarray experiment, though not those of the highly expressed genes, belong to the low concentration, linear part of the isotherm. Nevertheless, recognising the existence of the asymptotic saturation part of the isotherm is important for interpreting this distribution over the entire intensity range.
AB - We describe a physico-chemical model relating measured fluorescence intensities on oligonucleotide microarrays to the underlying specific target concentration in the hybridised solution via a hyperbolic isotherm response function. The model includes various chemical reactions occurring at the microarray surface and in bulk solution during hybridisation, including specific and non-specific hybridisation, and also the effects of probe-target dissociation during the post hybridisation washing phase. We analyse the distribution of fluorescence intensities for a complete microarray in the light of this physicochemical model. Our results indicate that the majority of signals in a typical microarray experiment, though not those of the highly expressed genes, belong to the low concentration, linear part of the isotherm. Nevertheless, recognising the existence of the asymptotic saturation part of the isotherm is important for interpreting this distribution over the entire intensity range.
UR - https://www.scopus.com/pages/publications/70450056994
U2 - 10.1109/CEC.2009.4983335
DO - 10.1109/CEC.2009.4983335
M3 - Conference Paper
SN - 9781424429592
T3 - 2009 IEEE Congress on Evolutionary Computation, CEC 2009
SP - 3095
EP - 3102
BT - 2009 IEEE Congress on Evolutionary Computation, CEC 2009
T2 - 2009 IEEE Congress on Evolutionary Computation, CEC 2009
Y2 - 18 May 2009 through 21 May 2009
ER -