Intramolecular DNA coiling mediated by metallo-supramolecular cylinders: Differential binding of P and M helical enantiomers

Isabelle Meistermann; Virtudes Moreno; Maria J. Prieto; Erlend Moldrheim; Einar Sletten; Syma Khalid; P. Mark Rodger; Jemma C. Peberdy; Christian J. Isaac; Alison Rodger; Michael J. Hannon

doi:10.1073/pnas.062634499

Intramolecular DNA coiling mediated by metallo-supramolecular cylinders: Differential binding of P and M helical enantiomers

Isabelle Meistermann, Virtudes Moreno, Maria J. Prieto, Erlend Moldrheim, Einar Sletten, Syma Khalid, P. Mark Rodger, Jemma C. Peberdy, Christian J. Isaac, Alison Rodger, Michael J. Hannon^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

185 Citations (Scopus)

Abstract

We have designed a synthetic tetracationic metallo-supramolecular cylinder that targets the major groove of DNA with a binding constant in excess of 10⁷ M^-1 and induces DNA bending and intramolecular coiling. The two enantiomers of the helical molecule bind differently to DNA and have different structural effects. We report the characterization of the interactions by a range of biophysical techniques. The M helical cylinder binds to the major groove and induces dramatic intramolecular coiling. The DNA bending is less dramatic for the P enantiomer.

Original language	English
Pages (from-to)	5069-5074
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	99
Issue number	8
DOIs	https://doi.org/10.1073/pnas.062634499
Publication status	Published - 16 Apr 2002
Externally published	Yes

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10.1073/pnas.062634499

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Meistermann, I., Moreno, V., Prieto, M. J., Moldrheim, E., Sletten, E., Khalid, S., Mark Rodger, P., Peberdy, J. C., Isaac, C. J., Rodger, A., & Hannon, M. J. (2002). Intramolecular DNA coiling mediated by metallo-supramolecular cylinders: Differential binding of P and M helical enantiomers. Proceedings of the National Academy of Sciences of the United States of America, 99(8), 5069-5074. https://doi.org/10.1073/pnas.062634499

@article{a104a48b379041968fd01ada503da4a5,

title = "Intramolecular DNA coiling mediated by metallo-supramolecular cylinders: Differential binding of P and M helical enantiomers",

abstract = "We have designed a synthetic tetracationic metallo-supramolecular cylinder that targets the major groove of DNA with a binding constant in excess of 107 M-1 and induces DNA bending and intramolecular coiling. The two enantiomers of the helical molecule bind differently to DNA and have different structural effects. We report the characterization of the interactions by a range of biophysical techniques. The M helical cylinder binds to the major groove and induces dramatic intramolecular coiling. The DNA bending is less dramatic for the P enantiomer.",

author = "Isabelle Meistermann and Virtudes Moreno and Prieto, {Maria J.} and Erlend Moldrheim and Einar Sletten and Syma Khalid and {Mark Rodger}, P. and Peberdy, {Jemma C.} and Isaac, {Christian J.} and Alison Rodger and Hannon, {Michael J.}",

year = "2002",

month = apr,

day = "16",

doi = "10.1073/pnas.062634499",

language = "English",

volume = "99",

pages = "5069--5074",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "8",

}

Meistermann, I, Moreno, V, Prieto, MJ, Moldrheim, E, Sletten, E, Khalid, S, Mark Rodger, P, Peberdy, JC, Isaac, CJ, Rodger, A & Hannon, MJ 2002, 'Intramolecular DNA coiling mediated by metallo-supramolecular cylinders: Differential binding of P and M helical enantiomers', Proceedings of the National Academy of Sciences of the United States of America, vol. 99, no. 8, pp. 5069-5074. https://doi.org/10.1073/pnas.062634499

Intramolecular DNA coiling mediated by metallo-supramolecular cylinders: Differential binding of P and M helical enantiomers. / Meistermann, Isabelle; Moreno, Virtudes; Prieto, Maria J. et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 8, 16.04.2002, p. 5069-5074.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Intramolecular DNA coiling mediated by metallo-supramolecular cylinders

T2 - Differential binding of P and M helical enantiomers

AU - Meistermann, Isabelle

AU - Moreno, Virtudes

AU - Prieto, Maria J.

AU - Moldrheim, Erlend

AU - Sletten, Einar

AU - Khalid, Syma

AU - Mark Rodger, P.

AU - Peberdy, Jemma C.

AU - Isaac, Christian J.

AU - Rodger, Alison

AU - Hannon, Michael J.

PY - 2002/4/16

Y1 - 2002/4/16

N2 - We have designed a synthetic tetracationic metallo-supramolecular cylinder that targets the major groove of DNA with a binding constant in excess of 107 M-1 and induces DNA bending and intramolecular coiling. The two enantiomers of the helical molecule bind differently to DNA and have different structural effects. We report the characterization of the interactions by a range of biophysical techniques. The M helical cylinder binds to the major groove and induces dramatic intramolecular coiling. The DNA bending is less dramatic for the P enantiomer.

AB - We have designed a synthetic tetracationic metallo-supramolecular cylinder that targets the major groove of DNA with a binding constant in excess of 107 M-1 and induces DNA bending and intramolecular coiling. The two enantiomers of the helical molecule bind differently to DNA and have different structural effects. We report the characterization of the interactions by a range of biophysical techniques. The M helical cylinder binds to the major groove and induces dramatic intramolecular coiling. The DNA bending is less dramatic for the P enantiomer.

UR - http://www.scopus.com/inward/record.url?scp=17444409376&partnerID=8YFLogxK

U2 - 10.1073/pnas.062634499

DO - 10.1073/pnas.062634499

M3 - Article

C2 - 11959957

AN - SCOPUS:17444409376

SN - 0027-8424

VL - 99

SP - 5069

EP - 5074

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 8

ER -

Intramolecular DNA coiling mediated by metallo-supramolecular cylinders: Differential binding of P and M helical enantiomers

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