Investigation of the properties of wild type and mutant alkaline phosphatase variants: A laboratory project linking genotype and phenotype

Susan M. Howitt*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    3 Citations (Scopus)

    Abstract

    An understanding of the link between genotype and phenotype is essential for biology students. A 3-wk laboratory project aimed at demonstrating this link and introducing early year students to some aspects of the research process is described. Students investigate the properties of wild type and mutant variants of alkaline phosphatase using the techniques of both biochemistry and molecular biology. Changes in enzyme activity are correlated with the changes in DNA sequence that introduce restriction enzyme sites. Mutants are also used to analyze the regulation of phoA gene expression. The application of different techniques to the same experimental system helps students to integrate information from different parts of the course.

    Original languageEnglish
    Pages (from-to)350-354
    Number of pages5
    JournalBiochemistry and Molecular Biology Education
    Volume35
    Issue number5
    DOIs
    Publication statusPublished - Sept 2007

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