Isolation and characterization of 15 polymorphic microsatellites in the Plethodontid salamander Ensatina eschscholtzii

Thomas J. Devitt; Ricardo Pereira; Lakshmi Jakkula; João Alexandrino; Carolyne Bardeleben; Craig Moritz

doi:10.1111/j.1755-0998.2009.02518.x

Isolation and characterization of 15 polymorphic microsatellites in the Plethodontid salamander Ensatina eschscholtzii

Thomas J. Devitt, Ricardo Pereira, Lakshmi Jakkula, João Alexandrino, Carolyne Bardeleben, Craig Moritz

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

We developed 15 new polymorphic microsatellites for the plethodontid salamander Ensatina eschscholtzii. Loci were isolated from a genomic library from Ensatina eschscholtzii xanthoptica enriched for (AAAG)_n repetitive elements. The number of alleles per locus ranged from 4 to 20 (mean 9) in the sampled population. Observed heterozygosity ranged from 0.37 to 1. None of the loci deviated from Hardy-Weinberg equilibrium or showed significant linkage disequilibrium after a Bonferroni correction for multiple comparisons. All loci amplified in the six other subspecies of the Ensatina eschscholtzii complex. These new markers will prove useful in measuring gene flow and population structure as well as patterns of mating and sperm use in Ensatina.

Original language	English
Pages (from-to)	966-969
Number of pages	4
Journal	Molecular Ecology Resources
Volume	9
Issue number	3
DOIs	https://doi.org/10.1111/j.1755-0998.2009.02518.x
Publication status	Published - May 2009
Externally published	Yes

Access to Document

10.1111/j.1755-0998.2009.02518.x

Cite this

@article{05cec24994994f9085d2e38ad398e126,

title = "Isolation and characterization of 15 polymorphic microsatellites in the Plethodontid salamander Ensatina eschscholtzii",

abstract = "We developed 15 new polymorphic microsatellites for the plethodontid salamander Ensatina eschscholtzii. Loci were isolated from a genomic library from Ensatina eschscholtzii xanthoptica enriched for (AAAG)n repetitive elements. The number of alleles per locus ranged from 4 to 20 (mean 9) in the sampled population. Observed heterozygosity ranged from 0.37 to 1. None of the loci deviated from Hardy-Weinberg equilibrium or showed significant linkage disequilibrium after a Bonferroni correction for multiple comparisons. All loci amplified in the six other subspecies of the Ensatina eschscholtzii complex. These new markers will prove useful in measuring gene flow and population structure as well as patterns of mating and sperm use in Ensatina.",

keywords = "Ensatina, Microsatellite, Plethodontid, Plethodontidae, Ring species, Salamander",

author = "Devitt, {Thomas J.} and Ricardo Pereira and Lakshmi Jakkula and Jo{\~a}o Alexandrino and Carolyne Bardeleben and Craig Moritz",

year = "2009",

month = may,

doi = "10.1111/j.1755-0998.2009.02518.x",

language = "English",

volume = "9",

pages = "966--969",

journal = "Molecular Ecology Resources",

issn = "1755-098X",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "3",

}

TY - JOUR

T1 - Isolation and characterization of 15 polymorphic microsatellites in the Plethodontid salamander Ensatina eschscholtzii

AU - Devitt, Thomas J.

AU - Pereira, Ricardo

AU - Jakkula, Lakshmi

AU - Alexandrino, João

AU - Bardeleben, Carolyne

AU - Moritz, Craig

PY - 2009/5

Y1 - 2009/5

N2 - We developed 15 new polymorphic microsatellites for the plethodontid salamander Ensatina eschscholtzii. Loci were isolated from a genomic library from Ensatina eschscholtzii xanthoptica enriched for (AAAG)n repetitive elements. The number of alleles per locus ranged from 4 to 20 (mean 9) in the sampled population. Observed heterozygosity ranged from 0.37 to 1. None of the loci deviated from Hardy-Weinberg equilibrium or showed significant linkage disequilibrium after a Bonferroni correction for multiple comparisons. All loci amplified in the six other subspecies of the Ensatina eschscholtzii complex. These new markers will prove useful in measuring gene flow and population structure as well as patterns of mating and sperm use in Ensatina.

AB - We developed 15 new polymorphic microsatellites for the plethodontid salamander Ensatina eschscholtzii. Loci were isolated from a genomic library from Ensatina eschscholtzii xanthoptica enriched for (AAAG)n repetitive elements. The number of alleles per locus ranged from 4 to 20 (mean 9) in the sampled population. Observed heterozygosity ranged from 0.37 to 1. None of the loci deviated from Hardy-Weinberg equilibrium or showed significant linkage disequilibrium after a Bonferroni correction for multiple comparisons. All loci amplified in the six other subspecies of the Ensatina eschscholtzii complex. These new markers will prove useful in measuring gene flow and population structure as well as patterns of mating and sperm use in Ensatina.

KW - Ensatina

KW - Microsatellite

KW - Plethodontid

KW - Plethodontidae

KW - Ring species

KW - Salamander

UR - http://www.scopus.com/inward/record.url?scp=65249168756&partnerID=8YFLogxK

U2 - 10.1111/j.1755-0998.2009.02518.x

DO - 10.1111/j.1755-0998.2009.02518.x

M3 - Article

SN - 1755-098X

VL - 9

SP - 966

EP - 969

JO - Molecular Ecology Resources

JF - Molecular Ecology Resources

IS - 3

ER -

Isolation and characterization of 15 polymorphic microsatellites in the Plethodontid salamander Ensatina eschscholtzii

Abstract

Access to Document

Other files and links

Fingerprint

Cite this