Isolation and characterization of microsatellite loci from the barley scald pathogen, Rhynchosporium secalis

Celeste C. Linde; Marcello Zala; Bruce A. McDonald

doi:10.1111/j.1471-8286.2005.00983.x

Isolation and characterization of microsatellite loci from the barley scald pathogen, Rhynchosporium secalis

Celeste C. Linde^*, Marcello Zala, Bruce A. McDonald

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Fifteen primer pairs were designed for 14 polymorphic microsatellite loci, which were isolated and characterized from genomic libraries of Rhynchosporium secalis. Conditions for multiplexing and simultaneous genotyping of up to eight loci in a single run are described. The number of alleles per polymorphic locus ranged from two to 13 in populations from Switzerland and Australia. Genotypic diversity ranged from 61.5 to 66.7. Gene diversity ranged from 0.08 to 0.89 for individual polymorphic loci, with averages of 0.54 and 0.62 for the populations from Switzerland and Australia, respectively. Variable levels of polymorphism make these ideal markers for population genetic analyses.

Original language	English
Pages (from-to)	546-548
Number of pages	3
Journal	Molecular Ecology Notes
Volume	5
Issue number	3
DOIs	https://doi.org/10.1111/j.1471-8286.2005.00983.x
Publication status	Published - Sept 2005

Access to Document

10.1111/j.1471-8286.2005.00983.x

Cite this

@article{da72c5ed52624a20b49caa27c11bc48e,

title = "Isolation and characterization of microsatellite loci from the barley scald pathogen, Rhynchosporium secalis",

abstract = "Fifteen primer pairs were designed for 14 polymorphic microsatellite loci, which were isolated and characterized from genomic libraries of Rhynchosporium secalis. Conditions for multiplexing and simultaneous genotyping of up to eight loci in a single run are described. The number of alleles per polymorphic locus ranged from two to 13 in populations from Switzerland and Australia. Genotypic diversity ranged from 61.5 to 66.7. Gene diversity ranged from 0.08 to 0.89 for individual polymorphic loci, with averages of 0.54 and 0.62 for the populations from Switzerland and Australia, respectively. Variable levels of polymorphism make these ideal markers for population genetic analyses.",

keywords = "Barley scald, Genetic variation, Microsatellites, Population structure, Rhynchosporium secalis",

author = "Linde, {Celeste C.} and Marcello Zala and McDonald, {Bruce A.}",

year = "2005",

month = sep,

doi = "10.1111/j.1471-8286.2005.00983.x",

language = "English",

volume = "5",

pages = "546--548",

journal = "Molecular Ecology Notes",

issn = "1471-8278",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "3",

}

TY - JOUR

T1 - Isolation and characterization of microsatellite loci from the barley scald pathogen, Rhynchosporium secalis

AU - Linde, Celeste C.

AU - Zala, Marcello

AU - McDonald, Bruce A.

PY - 2005/9

Y1 - 2005/9

N2 - Fifteen primer pairs were designed for 14 polymorphic microsatellite loci, which were isolated and characterized from genomic libraries of Rhynchosporium secalis. Conditions for multiplexing and simultaneous genotyping of up to eight loci in a single run are described. The number of alleles per polymorphic locus ranged from two to 13 in populations from Switzerland and Australia. Genotypic diversity ranged from 61.5 to 66.7. Gene diversity ranged from 0.08 to 0.89 for individual polymorphic loci, with averages of 0.54 and 0.62 for the populations from Switzerland and Australia, respectively. Variable levels of polymorphism make these ideal markers for population genetic analyses.

AB - Fifteen primer pairs were designed for 14 polymorphic microsatellite loci, which were isolated and characterized from genomic libraries of Rhynchosporium secalis. Conditions for multiplexing and simultaneous genotyping of up to eight loci in a single run are described. The number of alleles per polymorphic locus ranged from two to 13 in populations from Switzerland and Australia. Genotypic diversity ranged from 61.5 to 66.7. Gene diversity ranged from 0.08 to 0.89 for individual polymorphic loci, with averages of 0.54 and 0.62 for the populations from Switzerland and Australia, respectively. Variable levels of polymorphism make these ideal markers for population genetic analyses.

KW - Barley scald

KW - Genetic variation

KW - Microsatellites

KW - Population structure

KW - Rhynchosporium secalis

UR - http://www.scopus.com/inward/record.url?scp=28344433794&partnerID=8YFLogxK

U2 - 10.1111/j.1471-8286.2005.00983.x

DO - 10.1111/j.1471-8286.2005.00983.x

M3 - Article

SN - 1471-8278

VL - 5

SP - 546

EP - 548

JO - Molecular Ecology Notes

JF - Molecular Ecology Notes

IS - 3

ER -

Isolation and characterization of microsatellite loci from the barley scald pathogen, Rhynchosporium secalis

Abstract

Access to Document

Other files and links

Fingerprint

Cite this