Isolation and characterization of novel microsatellite markers from the Australian water skink Eulamprus kosciuskoi and cross-species amplification in other members of the species-group

Ian A.W. Scott; Christine M. Hayes; J. Scott Keogh; Suzanne F. Morrison

doi:10.1046/j.1471-8278.2000.00011.x

Isolation and characterization of novel microsatellite markers from the Australian water skink Eulamprus kosciuskoi and cross-species amplification in other members of the species-group

Ian A.W. Scott, Christine M. Hayes, J. Scott Keogh^*, Suzanne F. Morrison

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

A panel of six samples from three Eulamprus species was used to screen 24 primer pairs developed from the Australian water skink Eulamprus kosciuskoi. We provide details of 10 microsatellite loci that exhibited the cleanest and strongest banding patterns. We also screened six of these microsatellite loci against 118 individuals from a population of E. heatwolei. These microsatellites exhibited large numbers of alleles per locus (4-19) and high heterozygosity (0.271-0.898) in this population. This suggests that they will be extremely useful for investigating mating systems and other facets of population biology in these lizards.

Original language	English
Pages (from-to)	28-30
Number of pages	3
Journal	Molecular Ecology Notes
Volume	1
Issue number	1-2
DOIs	https://doi.org/10.1046/j.1471-8278.2000.00011.x
Publication status	Published - 2001

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10.1046/j.1471-8278.2000.00011.x

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title = "Isolation and characterization of novel microsatellite markers from the Australian water skink Eulamprus kosciuskoi and cross-species amplification in other members of the species-group",

abstract = "A panel of six samples from three Eulamprus species was used to screen 24 primer pairs developed from the Australian water skink Eulamprus kosciuskoi. We provide details of 10 microsatellite loci that exhibited the cleanest and strongest banding patterns. We also screened six of these microsatellite loci against 118 individuals from a population of E. heatwolei. These microsatellites exhibited large numbers of alleles per locus (4-19) and high heterozygosity (0.271-0.898) in this population. This suggests that they will be extremely useful for investigating mating systems and other facets of population biology in these lizards.",

keywords = "Eulamprus, Lizard, Mating system, Microsatellite, Sphenomorphus group",

author = "Scott, {Ian A.W.} and Hayes, {Christine M.} and Keogh, {J. Scott} and Morrison, {Suzanne F.}",

year = "2001",

doi = "10.1046/j.1471-8278.2000.00011.x",

language = "English",

volume = "1",

pages = "28--30",

journal = "Molecular Ecology Notes",

issn = "1471-8278",

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Isolation and characterization of novel microsatellite markers from the Australian water skink Eulamprus kosciuskoi and cross-species amplification in other members of the species-group. / Scott, Ian A.W.; Hayes, Christine M.; Keogh, J. Scott et al.
In: Molecular Ecology Notes, Vol. 1, No. 1-2, 2001, p. 28-30.

Research output: Contribution to journal › Article › peer-review

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T1 - Isolation and characterization of novel microsatellite markers from the Australian water skink Eulamprus kosciuskoi and cross-species amplification in other members of the species-group

AU - Scott, Ian A.W.

AU - Hayes, Christine M.

AU - Keogh, J. Scott

AU - Morrison, Suzanne F.

PY - 2001

Y1 - 2001

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AB - A panel of six samples from three Eulamprus species was used to screen 24 primer pairs developed from the Australian water skink Eulamprus kosciuskoi. We provide details of 10 microsatellite loci that exhibited the cleanest and strongest banding patterns. We also screened six of these microsatellite loci against 118 individuals from a population of E. heatwolei. These microsatellites exhibited large numbers of alleles per locus (4-19) and high heterozygosity (0.271-0.898) in this population. This suggests that they will be extremely useful for investigating mating systems and other facets of population biology in these lizards.

KW - Eulamprus

KW - Lizard

KW - Mating system

KW - Microsatellite

KW - Sphenomorphus group

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DO - 10.1046/j.1471-8278.2000.00011.x

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VL - 1

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EP - 30

JO - Molecular Ecology Notes

JF - Molecular Ecology Notes

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Isolation and characterization of novel microsatellite markers from the Australian water skink Eulamprus kosciuskoi and cross-species amplification in other members of the species-group

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