Isolation and characterization of novel microsatellite markers from the Australian tiger snakes (Elapidae: Notechis) and amplification in the closely related genus Hoplocephalus

Ian A.W. Scott; Christine M. Hayes; J. Scott Keogh; Jonathon K. Webb

doi:10.1046/j.1471-8278.2001.00037.x

Isolation and characterization of novel microsatellite markers from the Australian tiger snakes (Elapidae: Notechis) and amplification in the closely related genus Hoplocephalus

Ian A.W. Scott, Christine M. Hayes, J. Scott Keogh^*, Jonathon K. Webb

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

We provide details of seven microsatellite loci (out of 20 primer pairs designed) that exhibited the cleanest and strongest banding patterns for the Eastern tiger snake Notechis scutatus. These loci were used to screen 76 individuals from across the geographical range of N. scutatus and a further 14 individuals of the closely related and endangered broadheaded snake Hoplocephalus bungaroides. We observed large numbers of alleles per locus (14-52) and relatively high levels of heterozygosity (0.270-0.696) within the N. scutatus sample. These markers are also likely to be informative for work on H. bungaroides.

Original language	English
Pages (from-to)	117-119
Number of pages	3
Journal	Molecular Ecology Notes
Volume	1
Issue number	3
DOIs	https://doi.org/10.1046/j.1471-8278.2001.00037.x
Publication status	Published - Sept 2001

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10.1046/j.1471-8278.2001.00037.x

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title = "Isolation and characterization of novel microsatellite markers from the Australian tiger snakes (Elapidae: Notechis) and amplification in the closely related genus Hoplocephalus",

abstract = "We provide details of seven microsatellite loci (out of 20 primer pairs designed) that exhibited the cleanest and strongest banding patterns for the Eastern tiger snake Notechis scutatus. These loci were used to screen 76 individuals from across the geographical range of N. scutatus and a further 14 individuals of the closely related and endangered broadheaded snake Hoplocephalus bungaroides. We observed large numbers of alleles per locus (14-52) and relatively high levels of heterozygosity (0.270-0.696) within the N. scutatus sample. These markers are also likely to be informative for work on H. bungaroides.",

keywords = "Elapid, Hoplocephalus, Microsatellite, Notechis, Snake",

author = "Scott, {Ian A.W.} and Hayes, {Christine M.} and Keogh, {J. Scott} and Webb, {Jonathon K.}",

year = "2001",

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doi = "10.1046/j.1471-8278.2001.00037.x",

language = "English",

volume = "1",

pages = "117--119",

journal = "Molecular Ecology Notes",

issn = "1471-8278",

publisher = "Wiley-Blackwell Publishing Ltd",

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}

Isolation and characterization of novel microsatellite markers from the Australian tiger snakes (Elapidae: Notechis) and amplification in the closely related genus Hoplocephalus. / Scott, Ian A.W.; Hayes, Christine M.; Keogh, J. Scott et al.
In: Molecular Ecology Notes, Vol. 1, No. 3, 09.2001, p. 117-119.

Research output: Contribution to journal › Article › peer-review

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T1 - Isolation and characterization of novel microsatellite markers from the Australian tiger snakes (Elapidae: Notechis) and amplification in the closely related genus Hoplocephalus

AU - Scott, Ian A.W.

AU - Hayes, Christine M.

AU - Keogh, J. Scott

AU - Webb, Jonathon K.

PY - 2001/9

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AB - We provide details of seven microsatellite loci (out of 20 primer pairs designed) that exhibited the cleanest and strongest banding patterns for the Eastern tiger snake Notechis scutatus. These loci were used to screen 76 individuals from across the geographical range of N. scutatus and a further 14 individuals of the closely related and endangered broadheaded snake Hoplocephalus bungaroides. We observed large numbers of alleles per locus (14-52) and relatively high levels of heterozygosity (0.270-0.696) within the N. scutatus sample. These markers are also likely to be informative for work on H. bungaroides.

KW - Elapid

KW - Hoplocephalus

KW - Microsatellite

KW - Notechis

KW - Snake

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DO - 10.1046/j.1471-8278.2001.00037.x

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VL - 1

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JO - Molecular Ecology Notes

JF - Molecular Ecology Notes

IS - 3

ER -

Isolation and characterization of novel microsatellite markers from the Australian tiger snakes (Elapidae: Notechis) and amplification in the closely related genus Hoplocephalus

Abstract

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