Label-free optical scattering and interferometry microscopy for functional imaging of thrombus

Yujie Zheng, Woei Ming Lee*

*Corresponding author for this work

    Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

    Abstract

    Living cells adjust their cytoskeletal organization and mechanically change their overall shape by reacting to the changes of the microenvironment. The ability to quantify these dynamic events in micro and nanoscale in real-Time at the same time contributes to our understanding of the functional response of living cells. The combination to achieve both microscale and nanoscale imaging simultaneous at volumetric speeds is challenging. Traditional TIRF microscopy has excelled in measuring surface interaction but yet limited in imaging depth and requires fluorescent labelling. Likewise, the ability to quantify the total volume and shape change of biological cells as they interact requires either confocal microscopy or lightsheet microscopy. In this paper, we propose an in toto label free approach through coherent optical interference to measure volumetric information and surface interaction at the same time to provide a full view of the cell during dynamic activities.

    Original languageEnglish
    Title of host publicationBiophotonics Australasia 2019
    EditorsEwa M. Goldys, Brant C. Gibson
    PublisherSPIE
    ISBN (Electronic)9781510631441
    DOIs
    Publication statusPublished - 2019
    EventBiophotonics Australasia 2019 - Melbourne, Australia
    Duration: 9 Dec 201912 Dec 2019

    Publication series

    NameProceedings of SPIE - The International Society for Optical Engineering
    Volume11202
    ISSN (Print)0277-786X
    ISSN (Electronic)1996-756X

    Conference

    ConferenceBiophotonics Australasia 2019
    Country/TerritoryAustralia
    CityMelbourne
    Period9/12/1912/12/19

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