LD spectroscopy of natural and synthetic biomaterials

Matthew R. Hicks, Jarosav Kowalski, Alison Rodger*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

59 Citations (Scopus)


The structural characterization of biomaterials is challenging because they are usually too large for NMR or high resolution mass spectrometry and not well-enough structured for X-ray crystallography. Structural characterization and kinetic analysis for such systems thus has to proceed by collecting complementary data from a wide range of different techniques. This tutorial review describes how linear dichroism, a polarized absorbance spectroscopy technique applied to oriented molecular systems, can be used to provide useful data on biomaterials. In particular LD can provide information about relative orientations of sub-units of biomaterials and orientations of the whole biomaterial with respect to an orientation axis. An outline of linear dichroism and a summary of the artifacts to be avoided are followed by a description of how Couette flow linear dichroism has been used for a range of biomaterial systems including: DNA; DNA:ligand systems; cytoskeletal fibrous proteins; synthetic protein fibres; membrane proteins in liposomes; bacteriophage; carbon nanotubes; and peptidoglycan systems.

Original languageEnglish
Pages (from-to)3380-3393
Number of pages14
JournalChemical Society Reviews
Issue number9
Publication statusPublished - 18 Aug 2010
Externally publishedYes


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