Leader sequences of eukaryotic mRNA can be simultaneously bound to initiating 80S ribosome and 40S ribosomal subunit

E. A. Sogorin; N. E. Shirokikh; A. M. Ibragimova; V. D. Vasiliev; S. Ch Agalarov; A. S. Spirin

doi:10.1134/S0006297912040049

Leader sequences of eukaryotic mRNA can be simultaneously bound to initiating 80S ribosome and 40S ribosomal subunit

E. A. Sogorin, N. E. Shirokikh, A. M. Ibragimova, V. D. Vasiliev, S. Ch Agalarov, A. S. Spirin^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Binding of mRNA leader sequences to ribosomes was studied in conditions of a cell-free translation system based on wheat germ extract. Leader sequence of TMV mRNA (the so-called omega-RNA sequence) was able to bind simulta-neously 80S ribosome and 40S ribosomal subunit. It was found that nucleotide substitutions in omega-RNA resulting in destabilization of RNA structure have no effect on the complex formation with both 80S ribosome and 40S ribosomal sub-unit. Leader sequence of globin mRNA is also able to form a similar joint complex. It is supposed that the ability of mRNA leader sequences to bind simultaneously 80S ribosome and 40S subunit is independent of leader nature and may reflect pre-viously unknown eukaryotic mechanisms of translation initiation.

Original language	English
Pages (from-to)	342-345
Number of pages	4
Journal	Biochemistry (Moscow)
Volume	77
Issue number	4
DOIs	https://doi.org/10.1134/S0006297912040049
Publication status	Published - Apr 2012
Externally published	Yes

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title = "Leader sequences of eukaryotic mRNA can be simultaneously bound to initiating 80S ribosome and 40S ribosomal subunit",

abstract = "Binding of mRNA leader sequences to ribosomes was studied in conditions of a cell-free translation system based on wheat germ extract. Leader sequence of TMV mRNA (the so-called omega-RNA sequence) was able to bind simulta-neously 80S ribosome and 40S ribosomal subunit. It was found that nucleotide substitutions in omega-RNA resulting in destabilization of RNA structure have no effect on the complex formation with both 80S ribosome and 40S ribosomal sub-unit. Leader sequence of globin mRNA is also able to form a similar joint complex. It is supposed that the ability of mRNA leader sequences to bind simultaneously 80S ribosome and 40S subunit is independent of leader nature and may reflect pre-viously unknown eukaryotic mechanisms of translation initiation.",

keywords = "Electron microscopy of ribosomes, Initiation of protein biosynthesis, Omega-RNA, Ribosomal complexes",

author = "Sogorin, {E. A.} and Shirokikh, {N. E.} and Ibragimova, {A. M.} and Vasiliev, {V. D.} and Agalarov, {S. Ch} and Spirin, {A. S.}",

year = "2012",

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doi = "10.1134/S0006297912040049",

language = "English",

volume = "77",

pages = "342--345",

journal = "Biochemistry (Moscow)",

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TY - JOUR

T1 - Leader sequences of eukaryotic mRNA can be simultaneously bound to initiating 80S ribosome and 40S ribosomal subunit

AU - Sogorin, E. A.

AU - Shirokikh, N. E.

AU - Ibragimova, A. M.

AU - Vasiliev, V. D.

AU - Agalarov, S. Ch

AU - Spirin, A. S.

PY - 2012/4

Y1 - 2012/4

N2 - Binding of mRNA leader sequences to ribosomes was studied in conditions of a cell-free translation system based on wheat germ extract. Leader sequence of TMV mRNA (the so-called omega-RNA sequence) was able to bind simulta-neously 80S ribosome and 40S ribosomal subunit. It was found that nucleotide substitutions in omega-RNA resulting in destabilization of RNA structure have no effect on the complex formation with both 80S ribosome and 40S ribosomal sub-unit. Leader sequence of globin mRNA is also able to form a similar joint complex. It is supposed that the ability of mRNA leader sequences to bind simultaneously 80S ribosome and 40S subunit is independent of leader nature and may reflect pre-viously unknown eukaryotic mechanisms of translation initiation.

AB - Binding of mRNA leader sequences to ribosomes was studied in conditions of a cell-free translation system based on wheat germ extract. Leader sequence of TMV mRNA (the so-called omega-RNA sequence) was able to bind simulta-neously 80S ribosome and 40S ribosomal subunit. It was found that nucleotide substitutions in omega-RNA resulting in destabilization of RNA structure have no effect on the complex formation with both 80S ribosome and 40S ribosomal sub-unit. Leader sequence of globin mRNA is also able to form a similar joint complex. It is supposed that the ability of mRNA leader sequences to bind simultaneously 80S ribosome and 40S subunit is independent of leader nature and may reflect pre-viously unknown eukaryotic mechanisms of translation initiation.

KW - Electron microscopy of ribosomes

KW - Initiation of protein biosynthesis

KW - Omega-RNA

KW - Ribosomal complexes

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U2 - 10.1134/S0006297912040049

DO - 10.1134/S0006297912040049

M3 - Article

SN - 0006-2979

VL - 77

SP - 342

EP - 345

JO - Biochemistry (Moscow)

JF - Biochemistry (Moscow)

IS - 4

ER -

Leader sequences of eukaryotic mRNA can be simultaneously bound to initiating 80S ribosome and 40S ribosomal subunit

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