Limitations of plasmid vaccines to complex viruses: Selected myxoma virus antigens as DNA vaccines were not protective

Mathew M. Adams; Barbara H. Van Leeuwen; Peter J. Kerr

doi:10.1016/j.vaccine.2004.05.023

Limitations of plasmid vaccines to complex viruses: Selected myxoma virus antigens as DNA vaccines were not protective

Mathew M. Adams, Barbara H. Van Leeuwen, Peter J. Kerr

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Myxoma virus, a poxvirus of the genus Leporipoxvirus, is the causative agent of the disease myxomatosis which is highly lethal in European rabbits (Oryctolagus cuniculus). Current vaccines to protect against myxomatosis are either attenuated live strains of the virus or the antigenically related rabbit fibroma virus. We examined the immune response of outbred domestic rabbits to the individual myxoma virus antigens M055R, M073R, M115L and M121R, delivered as DNA vaccines co-expressing rabbit interleukin-2 or interleukin-4. M115L and M121R were also delivered simultaneously. None of the vaccine constructs were able to protect the rabbits from disease or reduce mortality after challenge with virulent myxoma virus, despite induction of antigen-specific cell-mediated and humoral immune responses.

Original language	English
Pages (from-to)	198-204
Number of pages	7
Journal	Vaccine
Volume	23
Issue number	2
DOIs	https://doi.org/10.1016/j.vaccine.2004.05.023
Publication status	Published - 25 Nov 2004

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10.1016/j.vaccine.2004.05.023

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title = "Limitations of plasmid vaccines to complex viruses: Selected myxoma virus antigens as DNA vaccines were not protective",

abstract = "Myxoma virus, a poxvirus of the genus Leporipoxvirus, is the causative agent of the disease myxomatosis which is highly lethal in European rabbits (Oryctolagus cuniculus). Current vaccines to protect against myxomatosis are either attenuated live strains of the virus or the antigenically related rabbit fibroma virus. We examined the immune response of outbred domestic rabbits to the individual myxoma virus antigens M055R, M073R, M115L and M121R, delivered as DNA vaccines co-expressing rabbit interleukin-2 or interleukin-4. M115L and M121R were also delivered simultaneously. None of the vaccine constructs were able to protect the rabbits from disease or reduce mortality after challenge with virulent myxoma virus, despite induction of antigen-specific cell-mediated and humoral immune responses.",

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T2 - Selected myxoma virus antigens as DNA vaccines were not protective

AU - Adams, Mathew M.

AU - Van Leeuwen, Barbara H.

AU - Kerr, Peter J.

PY - 2004/11/25

Y1 - 2004/11/25

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AB - Myxoma virus, a poxvirus of the genus Leporipoxvirus, is the causative agent of the disease myxomatosis which is highly lethal in European rabbits (Oryctolagus cuniculus). Current vaccines to protect against myxomatosis are either attenuated live strains of the virus or the antigenically related rabbit fibroma virus. We examined the immune response of outbred domestic rabbits to the individual myxoma virus antigens M055R, M073R, M115L and M121R, delivered as DNA vaccines co-expressing rabbit interleukin-2 or interleukin-4. M115L and M121R were also delivered simultaneously. None of the vaccine constructs were able to protect the rabbits from disease or reduce mortality after challenge with virulent myxoma virus, despite induction of antigen-specific cell-mediated and humoral immune responses.

KW - DNA vaccine

KW - Myxomatosis

KW - Poxvirus

KW - Rabbit

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JO - Vaccine

JF - Vaccine

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ER -

Limitations of plasmid vaccines to complex viruses: Selected myxoma virus antigens as DNA vaccines were not protective

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