Linear Dichroism Spectra - Measurement

Alison Rodger

doi:10.1007/978-3-642-16712-6_642

Linear Dichroism Spectra - Measurement

Alison Rodger^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Entry for encyclopedia/dictionary › peer-review

1 Citation (Scopus)

Abstract

Definition
Linear dichroism (LD) is the difference in absorption of light polarized parallel and perpendicular to an orientation axis. The component parts of an LD experiment are:

A source of linearly polarized light

A means of detecting how much light is absorbed

A way to change the relative orientations of sample and light beam polarization

A method of orienting the sample

Basic Characteristics
This section outlines what is needed for collecting LD data.

The Instrumentation
There are two main methods for measuring LD spectra. The one requiring less specialized equipment is the two-spectra method. In this case, one inserts a polarizer into a normal absorbance spectrometer, measures a spectrum, and then rotates either the polarizer or the sample. If the sample is rotated, care must be taken to have the same light intensity through the same part of the sample for both polarizations. If the polarizer is rotated care must be taken to ensure the same light intensity is present in both...

Original language	English
Title of host publication	Encyclopedia of Biophysics
Editors	Gordon C. K. Roberts
Place of Publication	Berlin, Heidelberg
Publisher	Springer Science+Business Media B.V.
Pages	1239-1244
Number of pages	6
ISBN (Electronic)	9783642167126
ISBN (Print)	9783642167119
DOIs	https://doi.org/10.1007/978-3-642-16712-6_642
Publication status	Published - 2013
Externally published	Yes

Access to Document

10.1007/978-3-642-16712-6_642

Cite this

@inbook{bdc6a27f5f0b4d87b5c10fb0bc0c1a7c,

title = "Linear Dichroism Spectra - Measurement",

abstract = "Definition Linear dichroism (LD) is the difference in absorption of light polarized parallel and perpendicular to an orientation axis. The component parts of an LD experiment are: A source of linearly polarized light A means of detecting how much light is absorbed A way to change the relative orientations of sample and light beam polarization A method of orienting the sample Basic Characteristics This section outlines what is needed for collecting LD data. The Instrumentation There are two main methods for measuring LD spectra. The one requiring less specialized equipment is the two-spectra method. In this case, one inserts a polarizer into a normal absorbance spectrometer, measures a spectrum, and then rotates either the polarizer or the sample. If the sample is rotated, care must be taken to have the same light intensity through the same part of the sample for both polarizations. If the polarizer is rotated care must be taken to ensure the same light intensity is present in both...",

author = "Alison Rodger",

year = "2013",

doi = "10.1007/978-3-642-16712-6\_642",

language = "English",

isbn = "9783642167119",

pages = "1239--1244",

editor = "Roberts, \{Gordon C. K.\}",

booktitle = "Encyclopedia of Biophysics",

publisher = "Springer Science+Business Media B.V.",

address = "Netherlands",

}

TY - CHAP

T1 - Linear Dichroism Spectra - Measurement

AU - Rodger, Alison

PY - 2013

Y1 - 2013

N2 - Definition Linear dichroism (LD) is the difference in absorption of light polarized parallel and perpendicular to an orientation axis. The component parts of an LD experiment are: A source of linearly polarized light A means of detecting how much light is absorbed A way to change the relative orientations of sample and light beam polarization A method of orienting the sample Basic Characteristics This section outlines what is needed for collecting LD data. The Instrumentation There are two main methods for measuring LD spectra. The one requiring less specialized equipment is the two-spectra method. In this case, one inserts a polarizer into a normal absorbance spectrometer, measures a spectrum, and then rotates either the polarizer or the sample. If the sample is rotated, care must be taken to have the same light intensity through the same part of the sample for both polarizations. If the polarizer is rotated care must be taken to ensure the same light intensity is present in both...

AB - Definition Linear dichroism (LD) is the difference in absorption of light polarized parallel and perpendicular to an orientation axis. The component parts of an LD experiment are: A source of linearly polarized light A means of detecting how much light is absorbed A way to change the relative orientations of sample and light beam polarization A method of orienting the sample Basic Characteristics This section outlines what is needed for collecting LD data. The Instrumentation There are two main methods for measuring LD spectra. The one requiring less specialized equipment is the two-spectra method. In this case, one inserts a polarizer into a normal absorbance spectrometer, measures a spectrum, and then rotates either the polarizer or the sample. If the sample is rotated, care must be taken to have the same light intensity through the same part of the sample for both polarizations. If the polarizer is rotated care must be taken to ensure the same light intensity is present in both...

UR - https://www.scopus.com/pages/publications/105011785621

U2 - 10.1007/978-3-642-16712-6_642

DO - 10.1007/978-3-642-16712-6_642

M3 - Entry for encyclopedia/dictionary

AN - SCOPUS:105011785621

SN - 9783642167119

SP - 1239

EP - 1244

BT - Encyclopedia of Biophysics

A2 - Roberts, Gordon C. K.

PB - Springer Science+Business Media B.V.

CY - Berlin, Heidelberg

ER -

Linear Dichroism Spectra - Measurement

Abstract

Access to Document

Other files and links

Fingerprint

Cite this