Macromolecular crowding and its influence on possible reaction mechanisms in photosynthetic electron flow

I. G. Tremmel; E. Weis; G. D. Farquhar

doi:10.1016/j.bbabio.2007.03.007

Macromolecular crowding and its influence on possible reaction mechanisms in photosynthetic electron flow

I. G. Tremmel^*, E. Weis, G. D. Farquhar

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The diffusion of plastoquinol and its binding to the Qo site of the cyt bf complex in the course of photosynthetic electron transport was studied by following the sigmoidal flash-induced re-reduction kinetics of P700 after previous oxidation of the intersystem electron carriers. The data resulting from these experiments were matched with a simulation of electron transport using Monte Carlo techniques. The simulation was able to account for the experimental observations. Two different extreme cases of reaction mechanism at the Qo site were compared: a diffusion limited collisional mechanism and a non-diffusion limited tight binding mechanism. Assuming a tight binding mechanism led to best matches due to the high protein density in thylakoids. The varied parameters resulted in values well within the range of published data. The results emphasise the importance of structural characteristics of thylakoids in models of electron transport.

Original language	English
Pages (from-to)	353-361
Number of pages	9
Journal	Biochimica et Biophysica Acta - Bioenergetics
Volume	1767
Issue number	5
DOIs	https://doi.org/10.1016/j.bbabio.2007.03.007
Publication status	Published - May 2007

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10.1016/j.bbabio.2007.03.007

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title = "Macromolecular crowding and its influence on possible reaction mechanisms in photosynthetic electron flow",

abstract = "The diffusion of plastoquinol and its binding to the Qo site of the cyt bf complex in the course of photosynthetic electron transport was studied by following the sigmoidal flash-induced re-reduction kinetics of P700 after previous oxidation of the intersystem electron carriers. The data resulting from these experiments were matched with a simulation of electron transport using Monte Carlo techniques. The simulation was able to account for the experimental observations. Two different extreme cases of reaction mechanism at the Qo site were compared: a diffusion limited collisional mechanism and a non-diffusion limited tight binding mechanism. Assuming a tight binding mechanism led to best matches due to the high protein density in thylakoids. The varied parameters resulted in values well within the range of published data. The results emphasise the importance of structural characteristics of thylakoids in models of electron transport.",

keywords = "Macromolecular crowding, Monte Carlo simulation, Photosynthesis, Plastoquinone diffusion, Reaction mechanism, Thylakoid membranes",

author = "Tremmel, {I. G.} and E. Weis and Farquhar, {G. D.}",

year = "2007",

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T1 - Macromolecular crowding and its influence on possible reaction mechanisms in photosynthetic electron flow

AU - Tremmel, I. G.

AU - Weis, E.

AU - Farquhar, G. D.

PY - 2007/5

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AB - The diffusion of plastoquinol and its binding to the Qo site of the cyt bf complex in the course of photosynthetic electron transport was studied by following the sigmoidal flash-induced re-reduction kinetics of P700 after previous oxidation of the intersystem electron carriers. The data resulting from these experiments were matched with a simulation of electron transport using Monte Carlo techniques. The simulation was able to account for the experimental observations. Two different extreme cases of reaction mechanism at the Qo site were compared: a diffusion limited collisional mechanism and a non-diffusion limited tight binding mechanism. Assuming a tight binding mechanism led to best matches due to the high protein density in thylakoids. The varied parameters resulted in values well within the range of published data. The results emphasise the importance of structural characteristics of thylakoids in models of electron transport.

KW - Macromolecular crowding

KW - Monte Carlo simulation

KW - Photosynthesis

KW - Plastoquinone diffusion

KW - Reaction mechanism

KW - Thylakoid membranes

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DO - 10.1016/j.bbabio.2007.03.007

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JO - Biochimica et Biophysica Acta - Bioenergetics

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IS - 5

ER -

Macromolecular crowding and its influence on possible reaction mechanisms in photosynthetic electron flow

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