TY - JOUR
T1 - Mapping the I-3 gene for resistance to Fusarium wilt in tomato
T2 - Application of an I-3 marker in tomato improvement and progress towards the cloning of I-3
AU - Lim, G. T.T.
AU - Wang, G. P.
AU - Hemming, M. N.
AU - Basuki, S.
AU - McGrath, D. J.
AU - Carroll, B. J.
AU - Jones, D. A.
PY - 2006
Y1 - 2006
N2 - Fusarium wilt of tomato, caused by the fungal pathogen, Fusarium oxysporum f. sp. lycopersici (Fol), is an economically damaging disease that results in huge losses in Australia and other countries worldwide. The I-3 gene, which confers resistance to Fol race 3, has been described in wild tomato, Lycopersicon pennellii, accessions LA716 and PI414773. We are pursuing the isolation of I-3 from LA716 by map-based cloning. We have constructed a high-resolution map of the I-3 region and have identified markers closely flanking I-3 as well as markers co-segregating with I-3. In addition, construction of a physical map based on these markers has been initiated. This review describes the context of our research and our progress towards isolating the I-3 gene. It also describes some important practical outcomes of our work, including the development and use of a PCR-based marker for marker-assisted selection for I-3, and the finding that the I-3 gene from LA716 is different to that from PI1414773, which we have now designated I-7. Tomato varieties combining I-3 and I-7 have been developed and are currently being introduced into commercial production to further safeguard tomato crops against Fusarium wilt.
AB - Fusarium wilt of tomato, caused by the fungal pathogen, Fusarium oxysporum f. sp. lycopersici (Fol), is an economically damaging disease that results in huge losses in Australia and other countries worldwide. The I-3 gene, which confers resistance to Fol race 3, has been described in wild tomato, Lycopersicon pennellii, accessions LA716 and PI414773. We are pursuing the isolation of I-3 from LA716 by map-based cloning. We have constructed a high-resolution map of the I-3 region and have identified markers closely flanking I-3 as well as markers co-segregating with I-3. In addition, construction of a physical map based on these markers has been initiated. This review describes the context of our research and our progress towards isolating the I-3 gene. It also describes some important practical outcomes of our work, including the development and use of a PCR-based marker for marker-assisted selection for I-3, and the finding that the I-3 gene from LA716 is different to that from PI1414773, which we have now designated I-7. Tomato varieties combining I-3 and I-7 have been developed and are currently being introduced into commercial production to further safeguard tomato crops against Fusarium wilt.
KW - Avirulence
KW - Recombination suppression
KW - Tomato chromosome 7
KW - Whole chromosome introgression
UR - http://www.scopus.com/inward/record.url?scp=33750548455&partnerID=8YFLogxK
U2 - 10.1071/AP06073
DO - 10.1071/AP06073
M3 - Article
SN - 0815-3191
VL - 35
SP - 671
EP - 680
JO - Australasian Plant Pathology
JF - Australasian Plant Pathology
IS - 6
ER -