Measurement of amyloid formation by turbidity assay—seeing through the cloud

Ran Zhao, Masatomo So, Hendrik Maat, Nicholas J. Ray, Fumio Arisaka, Yuji Goto, John A. Carver, Damien Hall*

*Corresponding author for this work

    Research output: Contribution to journalReview articlepeer-review

    62 Citations (Scopus)

    Abstract

    Detection of amyloid growth is commonly carried out by measurement of solution turbidity, a low-cost assay procedure based on the intrinsic light scattering properties of the protein aggregate. Here, we review the biophysical chemistry associated with the turbidimetric assay methodology, exploring the reviewed literature using a series of pedagogical kinetic simulations. In turn, these simulations are used to interrogate the literature concerned with in vitro drug screening and the assessment of amyloid aggregation mechanisms.

    Original languageEnglish
    Pages (from-to)445-471
    Number of pages27
    JournalBiophysical Reviews
    Volume8
    Issue number4
    DOIs
    Publication statusPublished - 1 Dec 2016

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