Measurement of amyloid formation by turbidity assay—seeing through the cloud

Ran Zhao; Masatomo So; Hendrik Maat; Nicholas J. Ray; Fumio Arisaka; Yuji Goto; John A. Carver; Damien Hall

doi:10.1007/s12551-016-0233-7

Measurement of amyloid formation by turbidity assay—seeing through the cloud

Ran Zhao, Masatomo So, Hendrik Maat, Nicholas J. Ray, Fumio Arisaka, Yuji Goto, John A. Carver, Damien Hall^*

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

69 Citations (Scopus)

Abstract

Detection of amyloid growth is commonly carried out by measurement of solution turbidity, a low-cost assay procedure based on the intrinsic light scattering properties of the protein aggregate. Here, we review the biophysical chemistry associated with the turbidimetric assay methodology, exploring the reviewed literature using a series of pedagogical kinetic simulations. In turn, these simulations are used to interrogate the literature concerned with in vitro drug screening and the assessment of amyloid aggregation mechanisms.

Original language	English
Pages (from-to)	445-471
Number of pages	27
Journal	Biophysical Reviews
Volume	8
Issue number	4
DOIs	https://doi.org/10.1007/s12551-016-0233-7
Publication status	Published - 1 Dec 2016

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title = "Measurement of amyloid formation by turbidity assay—seeing through the cloud",

abstract = "Detection of amyloid growth is commonly carried out by measurement of solution turbidity, a low-cost assay procedure based on the intrinsic light scattering properties of the protein aggregate. Here, we review the biophysical chemistry associated with the turbidimetric assay methodology, exploring the reviewed literature using a series of pedagogical kinetic simulations. In turn, these simulations are used to interrogate the literature concerned with in vitro drug screening and the assessment of amyloid aggregation mechanisms.",

keywords = "Amyloid aggregation kinetics, Amyloid biophysics, Data reduction, Nonlinear signal response, Turbidimetric method",

author = "Ran Zhao and Masatomo So and Hendrik Maat and Ray, {Nicholas J.} and Fumio Arisaka and Yuji Goto and Carver, {John A.} and Damien Hall",

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doi = "10.1007/s12551-016-0233-7",

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T1 - Measurement of amyloid formation by turbidity assay—seeing through the cloud

AU - Zhao, Ran

AU - So, Masatomo

AU - Maat, Hendrik

AU - Ray, Nicholas J.

AU - Arisaka, Fumio

AU - Goto, Yuji

AU - Carver, John A.

AU - Hall, Damien

PY - 2016/12/1

Y1 - 2016/12/1

N2 - Detection of amyloid growth is commonly carried out by measurement of solution turbidity, a low-cost assay procedure based on the intrinsic light scattering properties of the protein aggregate. Here, we review the biophysical chemistry associated with the turbidimetric assay methodology, exploring the reviewed literature using a series of pedagogical kinetic simulations. In turn, these simulations are used to interrogate the literature concerned with in vitro drug screening and the assessment of amyloid aggregation mechanisms.

AB - Detection of amyloid growth is commonly carried out by measurement of solution turbidity, a low-cost assay procedure based on the intrinsic light scattering properties of the protein aggregate. Here, we review the biophysical chemistry associated with the turbidimetric assay methodology, exploring the reviewed literature using a series of pedagogical kinetic simulations. In turn, these simulations are used to interrogate the literature concerned with in vitro drug screening and the assessment of amyloid aggregation mechanisms.

KW - Amyloid aggregation kinetics

KW - Amyloid biophysics

KW - Data reduction

KW - Nonlinear signal response

KW - Turbidimetric method

UR - http://www.scopus.com/inward/record.url?scp=85000538795&partnerID=8YFLogxK

U2 - 10.1007/s12551-016-0233-7

DO - 10.1007/s12551-016-0233-7

M3 - Review article

SN - 1867-2450

VL - 8

SP - 445

EP - 471

JO - Biophysical Reviews

JF - Biophysical Reviews

IS - 4

ER -

Measurement of amyloid formation by turbidity assay—seeing through the cloud

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