Measurement of glucose exclusion from the fully hydrated DOPE inverse hexagonal phase

Ben Kent, Christopher J. Garvey, Thomas Lenné, Lionel Porcar, Vasil M. Garamus, Gary Bryant*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    21 Citations (Scopus)

    Abstract

    The degree of exclusion of glucose from the inverse hexagonal H II phase of fully hydrated DOPE is determined using contrast variation small angle neutron scattering and small angle X-ray scattering. The presence of glucose is found to favour the formation of the non-lamellar H II phase over the fluid lamellar phase, over a wide range of temperatures, while having no significant effect on the structure of the H II phase. Glucose is preferentially excluded from the lipid-water interface resulting in a glucose concentration in the HII phase of less than half that in the coexisting aqueous phase. The degree of exclusion is quantified and the results are consistent with a hydration layer of pure water adjacent to the lipid head groups from which glucose is excluded. The osmotic gradient created by the difference in glucose concentration is determined and the influence of glucose on the phase behaviour of non-lamellar phase forming lipid systems is discussed.

    Original languageEnglish
    Pages (from-to)1197-1202
    Number of pages6
    JournalSoft Matter
    Volume6
    Issue number6
    DOIs
    Publication statusPublished - 2010

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