Measuring Solute Transport in Toxoplasma gondii Parasites

Esther Rajendran, Kiaran Kirk, Giel G. van Dooren*

*Corresponding author for this work

    Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

    3 Citations (Scopus)

    Abstract

    The uptake of host-derived nutrients is key to the growth and survival of Toxoplasma gondii parasites. Nutrients are acquired via solute transporters that localize to the plasma membrane of the parasites. In this chapter, we describe methodology by which the uptake of solutes via plasma membrane transporters may be monitored and characterized. These assays, used here to investigate the uptake of amino acids into parasites, have broad applicability in measuring the uptake of a diverse range of solutes.

    Original languageEnglish
    Title of host publicationMethods in Molecular Biology
    PublisherHumana Press Inc.
    Pages245-268
    Number of pages24
    DOIs
    Publication statusPublished - 2020

    Publication series

    NameMethods in Molecular Biology
    Volume2071
    ISSN (Print)1064-3745
    ISSN (Electronic)1940-6029

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