Metabolite profiling identifies the mycotoxin alternariol in the pathogen Stagonospora nodorum

Kar Chun Tan, Robert D. Trengove, Garth L. Maker, Richard P. Oliver, Peter S. Solomon*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    41 Citations (Scopus)

    Abstract

    A recent comparative proteomics study identified the short-chain dehydrogenase (Sch1) as being required for asexual sporulation (Tan et al. Eukaryotic Cell 7:1916-1929, 2008). Metabolite profiling was undertaken on the mutant strains of Stagonospora nodorum lacking the Sch1 gene to help elucidate its role. Gas chromatography-mass spectrometry of the polar metabolites in the Sch1 mutants identified a secondary metabolite at a 200-fold greater concentration than observed in the wild-type strains. Comparative analysis of the secondary metabolite and the mycotoxin alternariol using ESI-MS/MS confirmed the identity of the compound as alternariol. This is the first report to confirm the presence of a mycotoxin in S. nodorum and compelling the field to consider the health implication of this disease.

    Original languageEnglish
    Pages (from-to)330-335
    Number of pages6
    JournalMetabolomics
    Volume5
    Issue number3
    DOIs
    Publication statusPublished - 2009

    Fingerprint

    Dive into the research topics of 'Metabolite profiling identifies the mycotoxin alternariol in the pathogen Stagonospora nodorum'. Together they form a unique fingerprint.

    Cite this