Metabolite profiling identifies the mycotoxin alternariol in the pathogen Stagonospora nodorum

Kar Chun Tan; Robert D. Trengove; Garth L. Maker; Richard P. Oliver; Peter S. Solomon

doi:10.1007/s11306-009-0158-2

Metabolite profiling identifies the mycotoxin alternariol in the pathogen Stagonospora nodorum

Kar Chun Tan, Robert D. Trengove, Garth L. Maker, Richard P. Oliver, Peter S. Solomon^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

43 Citations (Scopus)

Abstract

A recent comparative proteomics study identified the short-chain dehydrogenase (Sch1) as being required for asexual sporulation (Tan et al. Eukaryotic Cell 7:1916-1929, 2008). Metabolite profiling was undertaken on the mutant strains of Stagonospora nodorum lacking the Sch1 gene to help elucidate its role. Gas chromatography-mass spectrometry of the polar metabolites in the Sch1 mutants identified a secondary metabolite at a 200-fold greater concentration than observed in the wild-type strains. Comparative analysis of the secondary metabolite and the mycotoxin alternariol using ESI-MS/MS confirmed the identity of the compound as alternariol. This is the first report to confirm the presence of a mycotoxin in S. nodorum and compelling the field to consider the health implication of this disease.

Original language	English
Pages (from-to)	330-335
Number of pages	6
Journal	Metabolomics
Volume	5
Issue number	3
DOIs	https://doi.org/10.1007/s11306-009-0158-2
Publication status	Published - 2009

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title = "Metabolite profiling identifies the mycotoxin alternariol in the pathogen Stagonospora nodorum",

abstract = "A recent comparative proteomics study identified the short-chain dehydrogenase (Sch1) as being required for asexual sporulation (Tan et al. Eukaryotic Cell 7:1916-1929, 2008). Metabolite profiling was undertaken on the mutant strains of Stagonospora nodorum lacking the Sch1 gene to help elucidate its role. Gas chromatography-mass spectrometry of the polar metabolites in the Sch1 mutants identified a secondary metabolite at a 200-fold greater concentration than observed in the wild-type strains. Comparative analysis of the secondary metabolite and the mycotoxin alternariol using ESI-MS/MS confirmed the identity of the compound as alternariol. This is the first report to confirm the presence of a mycotoxin in S. nodorum and compelling the field to consider the health implication of this disease.",

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AU - Trengove, Robert D.

AU - Maker, Garth L.

AU - Oliver, Richard P.

AU - Solomon, Peter S.

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AB - A recent comparative proteomics study identified the short-chain dehydrogenase (Sch1) as being required for asexual sporulation (Tan et al. Eukaryotic Cell 7:1916-1929, 2008). Metabolite profiling was undertaken on the mutant strains of Stagonospora nodorum lacking the Sch1 gene to help elucidate its role. Gas chromatography-mass spectrometry of the polar metabolites in the Sch1 mutants identified a secondary metabolite at a 200-fold greater concentration than observed in the wild-type strains. Comparative analysis of the secondary metabolite and the mycotoxin alternariol using ESI-MS/MS confirmed the identity of the compound as alternariol. This is the first report to confirm the presence of a mycotoxin in S. nodorum and compelling the field to consider the health implication of this disease.

KW - Alternariol

KW - Metabolomics

KW - Mycotoxin

KW - Pathogen

KW - Stagonospora nodorum

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DO - 10.1007/s11306-009-0158-2

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Metabolite profiling identifies the mycotoxin alternariol in the pathogen Stagonospora nodorum

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