Metabolite profiling of the intraerythrocytic malaria parasite Plasmodium falciparum by 1H NMR spectroscopy

Rongwei Teng, Pauline R. Junankar, William A. Bubb, Caroline Rae, Pascal Mercier, Kiaran Kirk*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    95 Citations (Scopus)

    Abstract

    NMR spectroscopy was used to identify and quantify compounds in extracts prepared from mature trophozoite-stage Plasmodium falciparum parasites isolated by saponin-permeabilisation of the host erythrocyte. One-dimensional 1H NMR spectroscopy and four two-dimensional NMR techniques were used to identify more than 50 metabolites. The intracellular concentrations of over 40 metabolites were estimated from the 1H NMR spectra of extracts prepared by four extraction methods: perchloric acid, methanol/water, methanol/chloroform/water, and methanol alone. The metabolites quantified included: the majority of the biological α-amino acids; 4-aminobutyric acid; mono-, di- and tri-carboxylic acids; nucleotides; polyamines; myo-inositol; and phosphocholine and phosphoethanolamine. The parasites also contained a significant concentration (up to 12 mM) of the exogenous buffering agent, HEPES. Although the metabolite profiles obtained with each extraction method were broadly similar, perchloric acid was found to have significant advantages over the other extraction media.

    Original languageEnglish
    Pages (from-to)292-302
    Number of pages11
    JournalNMR in Biomedicine
    Volume22
    Issue number3
    DOIs
    Publication statusPublished - 2009

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