Micro-CT-assisted determination of the shattering and non-shattering phenotypes of charred rice spikelet bases from archaeological sites: a new methodological approach

The selection of a non-shattering phenotype is a pivotal change in the process of rice domestication. However, current research is heavily restricted by the preservation conditions of macro-plant remains in early and middle Neolithic sites, as very limited well-preserved rice spikelet bases could be retrieved. We present a non-destructive method based on micro-computed tomographic (CT) scanning, which could provide detailed visualization of the internal structures of charred spikelet bases and efficiently discriminate the shattering and non-shattering phenotypes of rice spikelet bases according to the abundance of fusiform echinate phytoliths. It could be widely applied in different contexts, especially those poorly preserved specimens and tempers in pottery sherds, greatly improving our knowledge of rice domestication.