Microbial processes and carbon-isotope fractionation in tropical and temperate grassland soils

H. Šantrůčková*, M. I. Bird, J. Lloyd

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    192 Citations (Scopus)

    Abstract

    1. The carbon content and δ13C value of soil organic carbon (SOC), microbial biomass (C(mic)) and respired CO2 were measured in a range of grassland soils from tropical and temperate biomes to determine if isotope effect of microbial degradation can induce a shift in isotope composition of SOC and CO2. The soil from a depth of 0-2 cm was analysed. C(mic) was measured using the chloroform fumigation extraction method, while CO2 was measured in a closed system after 3 and 10 days of incubation. Two soils, temperate and tropical, were used for a long-term experiment, in which measurements were performed after 3, 10 and 40 days of incubation. 2. SOC and C(mic) decrease exponentially with increasing mean annual temperature. C(mic) decreases more slowly than SOC, resulting in a higher proportion of C(mic) in the SOC of tropical soils relative to temperate soils. 3. The δ13C value of C(mic) and respired CO2 reflects gross changes in the δ13C value of SOC in the corresponding sample. On average, C(mic) is 13C-enriched by c. 2‰ compared with SOC, while respired CO2 is 13C-depleted by c. 2.2‰ compared with C(mic). Thus, the observed 13C-enrichment in C(mic) is balanced by a corresponding 13C-depletion in respired CO2 resulting in the δ13C value of respired CO2 being approximately similar to the δ13C of SOC. 4. The isotope effect of microbial degradation is of importance in soil. It can be induced by selective utilization of SOC and isotope discrimination during metabolism. Metabolic isotopic discrimination is dependent on the growth stage of the soil microbial population.

    Original languageEnglish
    Pages (from-to)108-114
    Number of pages7
    JournalFunctional Ecology
    Volume14
    Issue number1
    DOIs
    Publication statusPublished - Feb 2000

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