MicroRNA-101 is a potent inhibitor of autophagy

Lisa B. Frankel, Jiayu Wen, Michael Lees, Maria Høyer-Hansen, Thomas Farkas, Anders Krogh, Marja Jäättelä, Anders H. Lund*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

308 Citations (Scopus)

Abstract

Autophagy is an evolutionarily conserved mechanism of cellular self-digestion in which proteins and organelles are degraded through delivery to lysosomes. Defects in this process are implicated in numerous human diseases including cancer. To further elucidate regulatory mechanisms of autophagy, we performed a functional screen in search of microRNAs (miRNAs), which regulate the autophagic flux in breast cancer cells. In this study, we identified the tumour suppressive miRNA, miR-101, as a potent inhibitor of basal, etoposide-and rapamycin-induced autophagy. Through transcriptome profiling, we identified three novel miR-101 targets, STMN1, RAB5A and ATG4D. siRNA-mediated depletion of these genes phenocopied the effect of miR-101 overexpression, demonstrating their importance in autophagy regulation. Importantly, overexpression of STMN1 could partially rescue cells from miR-101-mediated inhibition of autophagy, indicating a functional importance for this target. Finally, we show that miR-101-mediated inhibition of autophagy can sensitize breast cancer cells to 4-hydroxytamoxifen (4-OHT)-mediated cell death. Collectively, these data establish a novel link between two highly important and rapidly growing research fields and present a new role for miR-101 as a key regulator of autophagy.

Original languageEnglish
Pages (from-to)4628-4641
Number of pages14
JournalEMBO Journal
Volume30
Issue number22
DOIs
Publication statusPublished - 16 Nov 2011
Externally publishedYes

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