Mineralization of CaCO 3 in the presence of egg white lysozyme

Alina E. Voinescu; Didier Touraud; Alois Lecker; Arno Pfitzner; Werner Kunz; Barry W. Ninham

doi:10.1021/la701892v

Mineralization of CaCO ₃ in the presence of egg white lysozyme

Alina E. Voinescu
, Didier Touraud
, Alois Lecker
, Arno Pfitzner
, Werner Kunz^*
, Barry W. Ninham

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

53 Citations (Scopus)

Abstract

The influence of egg white lysozyme on the size, shape, crystallography, and chemical composition of amorphous calcium carbonate (ACC) particles obtained from aqueous CaCl ₂-dimethyl carbonate (DMC)-NaOH solutions was studied. At the onset of precipitation, the presence of lysozyme led to much smaller particles (50-400 nm spherical amorphous lysozyme-calcium carbonate particles (Ly-ACC)) than those obtained from lysozyme-free solution. The nanospheres were in some cases aggregated and in addition embedded in a faint network. Their size and interconnection depended on the concentration of egg white lysozyme. When the Ly-ACC particles were left in contact with the mother liquor (CaCl ₂/DMC/NaOH/ly sozyme solution) for 24 h, they transformed directly and exclusively into crystalline calcite. The observed results may be of relevance for a better understanding of the role of lysozyme in the process of eggshell mineralization.

Original language	English
Pages (from-to)	12269-12274
Number of pages	6
Journal	Langmuir
Volume	23
Issue number	24
DOIs	https://doi.org/10.1021/la701892v
Publication status	Published - 20 Nov 2007

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title = "Mineralization of CaCO 3 in the presence of egg white lysozyme",

abstract = "The influence of egg white lysozyme on the size, shape, crystallography, and chemical composition of amorphous calcium carbonate (ACC) particles obtained from aqueous CaCl 2-dimethyl carbonate (DMC)-NaOH solutions was studied. At the onset of precipitation, the presence of lysozyme led to much smaller particles (50-400 nm spherical amorphous lysozyme-calcium carbonate particles (Ly-ACC)) than those obtained from lysozyme-free solution. The nanospheres were in some cases aggregated and in addition embedded in a faint network. Their size and interconnection depended on the concentration of egg white lysozyme. When the Ly-ACC particles were left in contact with the mother liquor (CaCl 2/DMC/NaOH/ly sozyme solution) for 24 h, they transformed directly and exclusively into crystalline calcite. The observed results may be of relevance for a better understanding of the role of lysozyme in the process of eggshell mineralization.",

author = "Voinescu, \{Alina E.\} and Didier Touraud and Alois Lecker and Arno Pfitzner and Werner Kunz and Ninham, \{Barry W.\}",

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doi = "10.1021/la701892v",

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T1 - Mineralization of CaCO 3 in the presence of egg white lysozyme

AU - Voinescu, Alina E.

AU - Touraud, Didier

AU - Lecker, Alois

AU - Pfitzner, Arno

AU - Kunz, Werner

AU - Ninham, Barry W.

PY - 2007/11/20

Y1 - 2007/11/20

N2 - The influence of egg white lysozyme on the size, shape, crystallography, and chemical composition of amorphous calcium carbonate (ACC) particles obtained from aqueous CaCl 2-dimethyl carbonate (DMC)-NaOH solutions was studied. At the onset of precipitation, the presence of lysozyme led to much smaller particles (50-400 nm spherical amorphous lysozyme-calcium carbonate particles (Ly-ACC)) than those obtained from lysozyme-free solution. The nanospheres were in some cases aggregated and in addition embedded in a faint network. Their size and interconnection depended on the concentration of egg white lysozyme. When the Ly-ACC particles were left in contact with the mother liquor (CaCl 2/DMC/NaOH/ly sozyme solution) for 24 h, they transformed directly and exclusively into crystalline calcite. The observed results may be of relevance for a better understanding of the role of lysozyme in the process of eggshell mineralization.

AB - The influence of egg white lysozyme on the size, shape, crystallography, and chemical composition of amorphous calcium carbonate (ACC) particles obtained from aqueous CaCl 2-dimethyl carbonate (DMC)-NaOH solutions was studied. At the onset of precipitation, the presence of lysozyme led to much smaller particles (50-400 nm spherical amorphous lysozyme-calcium carbonate particles (Ly-ACC)) than those obtained from lysozyme-free solution. The nanospheres were in some cases aggregated and in addition embedded in a faint network. Their size and interconnection depended on the concentration of egg white lysozyme. When the Ly-ACC particles were left in contact with the mother liquor (CaCl 2/DMC/NaOH/ly sozyme solution) for 24 h, they transformed directly and exclusively into crystalline calcite. The observed results may be of relevance for a better understanding of the role of lysozyme in the process of eggshell mineralization.

UR - https://www.scopus.com/pages/publications/36649036339

U2 - 10.1021/la701892v

DO - 10.1021/la701892v

M3 - Article

SN - 0743-7463

VL - 23

SP - 12269

EP - 12274

JO - Langmuir

JF - Langmuir

IS - 24

ER -

Mineralization of CaCO ₃ in the presence of egg white lysozyme

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