Molecular basis of dark adaptation in rod photoreceptors

Following exposure of the eye to an intense light that ‘bleaches’ a significant fraction of the rhodopsin, one’s visual threshold is initially greatly elevated, and takes tens of minutes to recover to normal. The elevation of visual threshold arises from events occurring within the rod photoreceptors, and the underlying molecular basis of these events and of the rod’s recovery is now becoming clearer. Results obtained by exposing isolated toad rods to hydroxylamine solution indicate that, following small bleaches, the primary intermediate causing elevation of visual threshold is metarhodopsin II, in its phosphorylated and arrestin-bound form. This product activates transduction with an efficacy about 100 times greater than that of opsin.