Molecular cloning and expression analysis of a cDNA encoding a glutamate transporter in the honeybee brain

R. Kucharski, E. E. Ball, D. C. Hayward, R. Maleszka*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    52 Citations (Scopus)

    Abstract

    We have cloned and characterized a cDNA encoding a putative glutamate transporter, Am-EAAT, from the brain of the honeybee, Apis mellifera. The 543-amino-acid AmEAAT gene product shares the highest sequence identity (54%) with the human EAAT2 subtype. Am-EAAT is expressed predominantly in the brain, and its transcripts are abundant in the optic lobes and inner compact Kenyon cells of the mushroom bodies (MBs), with most other regions of the brain showing lower levels of Am-EAAT expression. High levels of Am-EAAT message are found in pupal stages, possibly indicating a role for glutamate in the developing brain. (C) 2000 Elsevier Science B.V. All rights reserved.

    Original languageEnglish
    Pages (from-to)399-405
    Number of pages7
    JournalGene
    Volume242
    Issue number1-2
    DOIs
    Publication statusPublished - 25 Jan 2000

    Fingerprint

    Dive into the research topics of 'Molecular cloning and expression analysis of a cDNA encoding a glutamate transporter in the honeybee brain'. Together they form a unique fingerprint.

    Cite this