Molecular cloning and expression analysis of a cDNA encoding a glutamate transporter in the honeybee brain

R. Kucharski; E. E. Ball; D. C. Hayward; R. Maleszka

doi:10.1016/S0378-1119(99)00503-X

Molecular cloning and expression analysis of a cDNA encoding a glutamate transporter in the honeybee brain

R. Kucharski, E. E. Ball, D. C. Hayward, R. Maleszka^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

53 Citations (Scopus)

Abstract

We have cloned and characterized a cDNA encoding a putative glutamate transporter, Am-EAAT, from the brain of the honeybee, Apis mellifera. The 543-amino-acid AmEAAT gene product shares the highest sequence identity (54%) with the human EAAT2 subtype. Am-EAAT is expressed predominantly in the brain, and its transcripts are abundant in the optic lobes and inner compact Kenyon cells of the mushroom bodies (MBs), with most other regions of the brain showing lower levels of Am-EAAT expression. High levels of Am-EAAT message are found in pupal stages, possibly indicating a role for glutamate in the developing brain. (C) 2000 Elsevier Science B.V. All rights reserved.

Original language	English
Pages (from-to)	399-405
Number of pages	7
Journal	Gene
Volume	242
Issue number	1-2
DOIs	https://doi.org/10.1016/S0378-1119(99)00503-X
Publication status	Published - 25 Jan 2000

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title = "Molecular cloning and expression analysis of a cDNA encoding a glutamate transporter in the honeybee brain",

abstract = "We have cloned and characterized a cDNA encoding a putative glutamate transporter, Am-EAAT, from the brain of the honeybee, Apis mellifera. The 543-amino-acid AmEAAT gene product shares the highest sequence identity (54%) with the human EAAT2 subtype. Am-EAAT is expressed predominantly in the brain, and its transcripts are abundant in the optic lobes and inner compact Kenyon cells of the mushroom bodies (MBs), with most other regions of the brain showing lower levels of Am-EAAT expression. High levels of Am-EAAT message are found in pupal stages, possibly indicating a role for glutamate in the developing brain. (C) 2000 Elsevier Science B.V. All rights reserved.",

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N2 - We have cloned and characterized a cDNA encoding a putative glutamate transporter, Am-EAAT, from the brain of the honeybee, Apis mellifera. The 543-amino-acid AmEAAT gene product shares the highest sequence identity (54%) with the human EAAT2 subtype. Am-EAAT is expressed predominantly in the brain, and its transcripts are abundant in the optic lobes and inner compact Kenyon cells of the mushroom bodies (MBs), with most other regions of the brain showing lower levels of Am-EAAT expression. High levels of Am-EAAT message are found in pupal stages, possibly indicating a role for glutamate in the developing brain. (C) 2000 Elsevier Science B.V. All rights reserved.

AB - We have cloned and characterized a cDNA encoding a putative glutamate transporter, Am-EAAT, from the brain of the honeybee, Apis mellifera. The 543-amino-acid AmEAAT gene product shares the highest sequence identity (54%) with the human EAAT2 subtype. Am-EAAT is expressed predominantly in the brain, and its transcripts are abundant in the optic lobes and inner compact Kenyon cells of the mushroom bodies (MBs), with most other regions of the brain showing lower levels of Am-EAAT expression. High levels of Am-EAAT message are found in pupal stages, possibly indicating a role for glutamate in the developing brain. (C) 2000 Elsevier Science B.V. All rights reserved.

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JO - Gene

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Molecular cloning and expression analysis of a cDNA encoding a glutamate transporter in the honeybee brain

Abstract

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