Molecular cloning of the mouse IMINO system: An Na+- and Cl --dependent proline transporter

Sonja Kowalczuk; Angelika Bröer; Michael Munzinger; Nadine Tietze; Karin Klingel; Stefan Bröer

doi:10.1042/BJ20050100

Molecular cloning of the mouse IMINO system: An Na⁺- and Cl ^--dependent proline transporter

Sonja Kowalczuk, Angelika Bröer, Michael Munzinger, Nadine Tietze, Karin Klingel, Stefan Bröer^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

96 Citations (Scopus)

Abstract

Neurotransmitter transporters of the SLC6 family play an important role in the removal of neurotransmitters in brain tissue and in amino acid transport in epithelial cells. Here we demonstrate that the mouse homologue of slc6a20 has all properties of the long-sought IMINO system. The mouse has two homologues corresponding to the single human SLC6A20 gene: these have been named XT3 and XT3s1. Expression of mouse XT3s1, but not XT3, in Xenopus laevis oocytes induced an electrogenic Na⁺-and-Cl^--dependent transporter for proline, hydroxyproline, betaine, N-methylaminoisobutyric acid and pipecolic acid. Expresssion of XT3s1 was found in brain, kidney, small intestine, thymus, spleen and lung, whereas XT3 prevailed in kidney and lung. Accordingly we suggest that the two homologues be termed 'XT3s1 IMINO^B' and 'XT3 IMINO^K' to indicate the tissue expression of the two genes.

Original language	English
Pages (from-to)	417-422
Number of pages	6
Journal	Biochemical Journal
Volume	386
Issue number	3
DOIs	https://doi.org/10.1042/BJ20050100
Publication status	Published - 15 Mar 2005

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title = "Molecular cloning of the mouse IMINO system: An Na+- and Cl --dependent proline transporter",

abstract = "Neurotransmitter transporters of the SLC6 family play an important role in the removal of neurotransmitters in brain tissue and in amino acid transport in epithelial cells. Here we demonstrate that the mouse homologue of slc6a20 has all properties of the long-sought IMINO system. The mouse has two homologues corresponding to the single human SLC6A20 gene: these have been named XT3 and XT3s1. Expression of mouse XT3s1, but not XT3, in Xenopus laevis oocytes induced an electrogenic Na+-and-Cl--dependent transporter for proline, hydroxyproline, betaine, N-methylaminoisobutyric acid and pipecolic acid. Expresssion of XT3s1 was found in brain, kidney, small intestine, thymus, spleen and lung, whereas XT3 prevailed in kidney and lung. Accordingly we suggest that the two homologues be termed 'XT3s1 IMINOB' and 'XT3 IMINOK' to indicate the tissue expression of the two genes.",

keywords = "Amino acid transport, Iminoglycinuria, Neurotransmitter transporter family, Orphan transporter, XT3, XTRP3",

author = "Sonja Kowalczuk and Angelika Br{\"o}er and Michael Munzinger and Nadine Tietze and Karin Klingel and Stefan Br{\"o}er",

year = "2005",

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doi = "10.1042/BJ20050100",

language = "English",

volume = "386",

pages = "417--422",

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T2 - An Na+- and Cl --dependent proline transporter

AU - Kowalczuk, Sonja

AU - Bröer, Angelika

AU - Munzinger, Michael

AU - Tietze, Nadine

AU - Klingel, Karin

AU - Bröer, Stefan

PY - 2005/3/15

Y1 - 2005/3/15

N2 - Neurotransmitter transporters of the SLC6 family play an important role in the removal of neurotransmitters in brain tissue and in amino acid transport in epithelial cells. Here we demonstrate that the mouse homologue of slc6a20 has all properties of the long-sought IMINO system. The mouse has two homologues corresponding to the single human SLC6A20 gene: these have been named XT3 and XT3s1. Expression of mouse XT3s1, but not XT3, in Xenopus laevis oocytes induced an electrogenic Na+-and-Cl--dependent transporter for proline, hydroxyproline, betaine, N-methylaminoisobutyric acid and pipecolic acid. Expresssion of XT3s1 was found in brain, kidney, small intestine, thymus, spleen and lung, whereas XT3 prevailed in kidney and lung. Accordingly we suggest that the two homologues be termed 'XT3s1 IMINOB' and 'XT3 IMINOK' to indicate the tissue expression of the two genes.

AB - Neurotransmitter transporters of the SLC6 family play an important role in the removal of neurotransmitters in brain tissue and in amino acid transport in epithelial cells. Here we demonstrate that the mouse homologue of slc6a20 has all properties of the long-sought IMINO system. The mouse has two homologues corresponding to the single human SLC6A20 gene: these have been named XT3 and XT3s1. Expression of mouse XT3s1, but not XT3, in Xenopus laevis oocytes induced an electrogenic Na+-and-Cl--dependent transporter for proline, hydroxyproline, betaine, N-methylaminoisobutyric acid and pipecolic acid. Expresssion of XT3s1 was found in brain, kidney, small intestine, thymus, spleen and lung, whereas XT3 prevailed in kidney and lung. Accordingly we suggest that the two homologues be termed 'XT3s1 IMINOB' and 'XT3 IMINOK' to indicate the tissue expression of the two genes.

KW - Amino acid transport

KW - Iminoglycinuria

KW - Neurotransmitter transporter family

KW - Orphan transporter

KW - XT3

KW - XTRP3

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U2 - 10.1042/BJ20050100

DO - 10.1042/BJ20050100

M3 - Article

SN - 0264-6021

VL - 386

SP - 417

EP - 422

JO - Biochemical Journal

JF - Biochemical Journal

IS - 3

ER -

Molecular cloning of the mouse IMINO system: An Na⁺- and Cl ^--dependent proline transporter

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