Molecular epidemiology of dengue fever outbreaks in Bhutan, 2016-2017

Sangay Zangmo; Jit Bdr Darnal; Sonam Gyeltshen; Binay Thapa Thapa; Prinyada Rodpradit; Piyawan Chinnawirotpisan; Wudtichai Manasatienkij; Louis R. Macareo; Stefan Fernandez; Sonam Wangchuk; Chonticha Klungthong

doi:10.1371/journal.pntd.0008165

Molecular epidemiology of dengue fever outbreaks in Bhutan, 2016-2017

Sangay Zangmo^*, Jit Bdr Darnal, Tsheten, Sonam Gyeltshen, Binay Thapa Thapa, Prinyada Rodpradit, Piyawan Chinnawirotpisan, Wudtichai Manasatienkij, Louis R. Macareo, Stefan Fernandez, Sonam Wangchuk, Chonticha Klungthong^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Dengue continues to pose a significant public health problem in tropical and subtropical countries. In Bhutan, first outbreak of dengue fever (DF) was reported in 2004 in a southern border town, followed by sporadic cases over the years. In this study, we analysed DF outbreaks that occurred in 3 different places during the years 2016 and 2017. A total of 533 cases in 2016 and 163 in 2017 were suspected of having of DF, where young adults were mostly affected. A total of 240 acute serum specimens collected and analyzed for serotype by nested RT-PCR revealed predominance of serotypes 1 and 2 (DENV-1 and 2). Phylogenetic analysis using envelope gene for both the serotypes demonstrated cosmopolitan genotype which were closely related to strains from India, indicating that they were probably imported from the neighboring country over the past few years.

Original language	English
Article number	e0008165
Pages (from-to)	1-12
Number of pages	12
Journal	PLoS Neglected Tropical Diseases
Volume	14
Issue number	4
DOIs	https://doi.org/10.1371/journal.pntd.0008165
Publication status	Published - Apr 2020
Externally published	Yes

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Zangmo, S., Darnal, J. B., Tsheten, Gyeltshen, S., Thapa, B. T., Rodpradit, P., Chinnawirotpisan, P., Manasatienkij, W., Macareo, L. R., Fernandez, S., Wangchuk, S., & Klungthong, C. (2020). Molecular epidemiology of dengue fever outbreaks in Bhutan, 2016-2017. PLoS Neglected Tropical Diseases, 14(4), 1-12. Article e0008165. https://doi.org/10.1371/journal.pntd.0008165

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title = "Molecular epidemiology of dengue fever outbreaks in Bhutan, 2016-2017",

abstract = "Dengue continues to pose a significant public health problem in tropical and subtropical countries. In Bhutan, first outbreak of dengue fever (DF) was reported in 2004 in a southern border town, followed by sporadic cases over the years. In this study, we analysed DF outbreaks that occurred in 3 different places during the years 2016 and 2017. A total of 533 cases in 2016 and 163 in 2017 were suspected of having of DF, where young adults were mostly affected. A total of 240 acute serum specimens collected and analyzed for serotype by nested RT-PCR revealed predominance of serotypes 1 and 2 (DENV-1 and 2). Phylogenetic analysis using envelope gene for both the serotypes demonstrated cosmopolitan genotype which were closely related to strains from India, indicating that they were probably imported from the neighboring country over the past few years.",

author = "Sangay Zangmo and Darnal, {Jit Bdr} and Tsheten and Sonam Gyeltshen and Thapa, {Binay Thapa} and Prinyada Rodpradit and Piyawan Chinnawirotpisan and Wudtichai Manasatienkij and Macareo, {Louis R.} and Stefan Fernandez and Sonam Wangchuk and Chonticha Klungthong",

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AU - Zangmo, Sangay

AU - Darnal, Jit Bdr

AU - Tsheten,

AU - Gyeltshen, Sonam

AU - Thapa, Binay Thapa

AU - Rodpradit, Prinyada

AU - Chinnawirotpisan, Piyawan

AU - Manasatienkij, Wudtichai

AU - Macareo, Louis R.

AU - Fernandez, Stefan

AU - Wangchuk, Sonam

AU - Klungthong, Chonticha

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AB - Dengue continues to pose a significant public health problem in tropical and subtropical countries. In Bhutan, first outbreak of dengue fever (DF) was reported in 2004 in a southern border town, followed by sporadic cases over the years. In this study, we analysed DF outbreaks that occurred in 3 different places during the years 2016 and 2017. A total of 533 cases in 2016 and 163 in 2017 were suspected of having of DF, where young adults were mostly affected. A total of 240 acute serum specimens collected and analyzed for serotype by nested RT-PCR revealed predominance of serotypes 1 and 2 (DENV-1 and 2). Phylogenetic analysis using envelope gene for both the serotypes demonstrated cosmopolitan genotype which were closely related to strains from India, indicating that they were probably imported from the neighboring country over the past few years.

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Molecular epidemiology of dengue fever outbreaks in Bhutan, 2016-2017

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