Molecular techniques and their limitations shape our view of the holobiont

Ira Cooke; Oliver Mead; Casey Whalen; Chloë Boote; Aurelie Moya; Hua Ying; Steven Robbins; Jan M. Strugnell; Aaron Darling; David Miller; Christian R. Voolstra; Maja Adamska

doi:10.1016/j.zool.2019.125695

Molecular techniques and their limitations shape our view of the holobiont

Ira Cooke^*, Oliver Mead, Casey Whalen, Chloë Boote, Aurelie Moya, Hua Ying, Steven Robbins, Jan M. Strugnell, Aaron Darling, David Miller, Christian R. Voolstra, Maja Adamska

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

7 Citations (Scopus)

Abstract

It is now recognised that the biology of almost any organism cannot be fully understood without recognising the existence and potential functional importance of associated microbes. Arguably, the emergence of this holistic viewpoint may never have occurred without the development of a crucial molecular technique, 16S rDNA amplicon sequencing, which allowed microbial communities to be easily profiled across a broad range of contexts. A diverse array of molecular techniques are now used to profile microbial communities, infer their evolutionary histories, visualise them in host tissues, and measure their molecular activity. In this review, we examine each of these categories of measurement and inference with a focus on the questions they make tractable, and the degree to which their capabilities and limitations shape our view of the holobiont.

Original language	English
Article number	125695
Journal	Zoology
Volume	137
DOIs	https://doi.org/10.1016/j.zool.2019.125695
Publication status	Published - Dec 2019

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title = "Molecular techniques and their limitations shape our view of the holobiont",

abstract = "It is now recognised that the biology of almost any organism cannot be fully understood without recognising the existence and potential functional importance of associated microbes. Arguably, the emergence of this holistic viewpoint may never have occurred without the development of a crucial molecular technique, 16S rDNA amplicon sequencing, which allowed microbial communities to be easily profiled across a broad range of contexts. A diverse array of molecular techniques are now used to profile microbial communities, infer their evolutionary histories, visualise them in host tissues, and measure their molecular activity. In this review, we examine each of these categories of measurement and inference with a focus on the questions they make tractable, and the degree to which their capabilities and limitations shape our view of the holobiont.",

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AU - Cooke, Ira

AU - Mead, Oliver

AU - Whalen, Casey

AU - Boote, Chloë

AU - Moya, Aurelie

AU - Ying, Hua

AU - Robbins, Steven

AU - Strugnell, Jan M.

AU - Darling, Aaron

AU - Miller, David

AU - Voolstra, Christian R.

AU - Adamska, Maja

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N2 - It is now recognised that the biology of almost any organism cannot be fully understood without recognising the existence and potential functional importance of associated microbes. Arguably, the emergence of this holistic viewpoint may never have occurred without the development of a crucial molecular technique, 16S rDNA amplicon sequencing, which allowed microbial communities to be easily profiled across a broad range of contexts. A diverse array of molecular techniques are now used to profile microbial communities, infer their evolutionary histories, visualise them in host tissues, and measure their molecular activity. In this review, we examine each of these categories of measurement and inference with a focus on the questions they make tractable, and the degree to which their capabilities and limitations shape our view of the holobiont.

AB - It is now recognised that the biology of almost any organism cannot be fully understood without recognising the existence and potential functional importance of associated microbes. Arguably, the emergence of this holistic viewpoint may never have occurred without the development of a crucial molecular technique, 16S rDNA amplicon sequencing, which allowed microbial communities to be easily profiled across a broad range of contexts. A diverse array of molecular techniques are now used to profile microbial communities, infer their evolutionary histories, visualise them in host tissues, and measure their molecular activity. In this review, we examine each of these categories of measurement and inference with a focus on the questions they make tractable, and the degree to which their capabilities and limitations shape our view of the holobiont.

KW - Evolutionary inference

KW - Holobiont

KW - Imaging

KW - Metagenomic sequencing

KW - Model system

KW - Multi-omics

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JO - Zoology

JF - Zoology

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ER -

Molecular techniques and their limitations shape our view of the holobiont

Abstract

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