Monitoring the purification by high‐performance liquid chromatography of cardiotoxins from Naja mossambica mossambica using phase‐sensitive two‐dimensional nuclear magnetic resonance

Gottfried OTTING; Pascale MARCHOT; Pierre E. BOUGIS; Hervé ROCHAT; Kurt WÜTHRICH

doi:10.1111/j.1432-1033.1987.tb13459.x

Monitoring the purification by high‐performance liquid chromatography of cardiotoxins from Naja mossambica mossambica using phase‐sensitive two‐dimensional nuclear magnetic resonance

Gottfried OTTING, Pascale MARCHOT, Pierre E. BOUGIS, Hervé ROCHAT, Kurt WÜTHRICH^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

High‐resolution phase‐sensitive two‐dimensional proton nuclear magnetic resonance was used to monitor the preparation by high‐performance liquid chromatography of homogeneous proteins from the venom of Naja mossambica mossambica. This resulted in the characterization of a heterogeneous protein preparation V^II2, which had been used in earlier structural studies by NMR, as well as a homogeneous protein CTXIIb and a nearly homogeneous protein fraction CTXIIa, which are now both subject to further investigations of their solution conformations.

Original language	English
Pages (from-to)	603-607
Number of pages	5
Journal	European Journal of Biochemistry
Volume	168
Issue number	3
DOIs	https://doi.org/10.1111/j.1432-1033.1987.tb13459.x
Publication status	Published - Nov 1987
Externally published	Yes

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title = "Monitoring the purification by high‐performance liquid chromatography of cardiotoxins from Naja mossambica mossambica using phase‐sensitive two‐dimensional nuclear magnetic resonance",

abstract = "High‐resolution phase‐sensitive two‐dimensional proton nuclear magnetic resonance was used to monitor the preparation by high‐performance liquid chromatography of homogeneous proteins from the venom of Naja mossambica mossambica. This resulted in the characterization of a heterogeneous protein preparation VII2, which had been used in earlier structural studies by NMR, as well as a homogeneous protein CTXIIb and a nearly homogeneous protein fraction CTXIIa, which are now both subject to further investigations of their solution conformations.",

author = "Gottfried OTTING and Pascale MARCHOT and BOUGIS, \{Pierre E.\} and Herv{\'e} ROCHAT and Kurt W{\"U}THRICH",

year = "1987",

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language = "English",

volume = "168",

pages = "603--607",

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OTTING, G, MARCHOT, P, BOUGIS, PE, ROCHAT, H & WÜTHRICH, K 1987, 'Monitoring the purification by high‐performance liquid chromatography of cardiotoxins from Naja mossambica mossambica using phase‐sensitive two‐dimensional nuclear magnetic resonance', European Journal of Biochemistry, vol. 168, no. 3, pp. 603-607. https://doi.org/10.1111/j.1432-1033.1987.tb13459.x

Monitoring the purification by high‐performance liquid chromatography of cardiotoxins from Naja mossambica mossambica using phase‐sensitive two‐dimensional nuclear magnetic resonance. / OTTING, Gottfried; MARCHOT, Pascale; BOUGIS, Pierre E. et al.
In: European Journal of Biochemistry, Vol. 168, No. 3, 11.1987, p. 603-607.

Research output: Contribution to journal › Article › peer-review

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AU - OTTING, Gottfried

AU - MARCHOT, Pascale

AU - BOUGIS, Pierre E.

AU - ROCHAT, Hervé

AU - WÜTHRICH, Kurt

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AB - High‐resolution phase‐sensitive two‐dimensional proton nuclear magnetic resonance was used to monitor the preparation by high‐performance liquid chromatography of homogeneous proteins from the venom of Naja mossambica mossambica. This resulted in the characterization of a heterogeneous protein preparation VII2, which had been used in earlier structural studies by NMR, as well as a homogeneous protein CTXIIb and a nearly homogeneous protein fraction CTXIIa, which are now both subject to further investigations of their solution conformations.

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Monitoring the purification by high‐performance liquid chromatography of cardiotoxins from Naja mossambica mossambica using phase‐sensitive two‐dimensional nuclear magnetic resonance

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